TY - JOUR
T1 - The DNA intermediate in yeast Ty1 element transposition copurifies with virus-like particles
T2 - Cell-free Ty1 transposition
AU - Eichinger, Daniel J.
AU - Boeke, Jef D.
N1 - Funding Information:
We thank P. Brown and L. Gerace for helpful discussions, S. Desiderio and R. Reed for comments on the manuscript, and G. Barcak. S. Goff, H. Murialdo, and 8. Seed for providing strains. We are grateful to S. Pelger for sequencing several of the Ty-x junctions. The work was supported by National Institutes of Health grant GM-38481 to J. D. B. D. J. E. was supported by PHS training grant CA09139 from the National Institutes of Health.
PY - 1988/9/23
Y1 - 1988/9/23
N2 - Yeast Ty1 elements are retrotransposons that transpose via an RNA intermediate found in a virus-like particle (Ty-VLP). A Ty-encoded reverse transcriptase activity found inside the particles is capable of giving rise to full-length reverse transcripts. The predominant form of these reverse transcripts is a full-length linear duplex DNA. We háve developed a cell-free system for transposition of Ty1 DNA molecules into a bacteriophage λ target. Purified Ty-VLPs and target DNA are the only macromolecular components required for the transposition reaction. A TYB-encoded protein, p90-TYB, contains amino acid sequences that are similar to those of retroviral integrase proteins. Mutations in the integrase coding region abolish transposition both in vivo and in vitro.
AB - Yeast Ty1 elements are retrotransposons that transpose via an RNA intermediate found in a virus-like particle (Ty-VLP). A Ty-encoded reverse transcriptase activity found inside the particles is capable of giving rise to full-length reverse transcripts. The predominant form of these reverse transcripts is a full-length linear duplex DNA. We háve developed a cell-free system for transposition of Ty1 DNA molecules into a bacteriophage λ target. Purified Ty-VLPs and target DNA are the only macromolecular components required for the transposition reaction. A TYB-encoded protein, p90-TYB, contains amino acid sequences that are similar to those of retroviral integrase proteins. Mutations in the integrase coding region abolish transposition both in vivo and in vitro.
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U2 - 10.1016/0092-8674(88)90110-9
DO - 10.1016/0092-8674(88)90110-9
M3 - Article
C2 - 2843295
AN - SCOPUS:0024295403
SN - 0092-8674
VL - 54
SP - 955
EP - 966
JO - Cell
JF - Cell
IS - 7
ER -