The DNA intermediate in yeast Ty1 element transposition copurifies with virus-like particles: Cell-free Ty1 transposition

Daniel J. Eichinger, Jef D. Boeke

    Research output: Contribution to journalArticle

    Abstract

    Yeast Ty1 elements are retrotransposons that transpose via an RNA intermediate found in a virus-like particle (Ty-VLP). A Ty-encoded reverse transcriptase activity found inside the particles is capable of giving rise to full-length reverse transcripts. The predominant form of these reverse transcripts is a full-length linear duplex DNA. We háve developed a cell-free system for transposition of Ty1 DNA molecules into a bacteriophage λ target. Purified Ty-VLPs and target DNA are the only macromolecular components required for the transposition reaction. A TYB-encoded protein, p90-TYB, contains amino acid sequences that are similar to those of retroviral integrase proteins. Mutations in the integrase coding region abolish transposition both in vivo and in vitro.

    Original languageEnglish (US)
    Pages (from-to)955-966
    Number of pages12
    JournalCell
    Volume54
    Issue number7
    DOIs
    StatePublished - Sep 23 1988

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

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