The Drosophila clock gene double-time encodes a protein closely related to human casein kinase Iε

Brian Kloss; Jeffrey L. Price; Lino Saez; Justin Blau; Adrian Rothenfluh; Cedric S. Wesley; Michael W. Young

doi:10.1016/S0092-8674(00)81225-8

The Drosophila clock gene double-time encodes a protein closely related to human casein kinase Iε

Brian Kloss, Jeffrey L. Price, Lino Saez, Justin Blau, Adrian Rothenfluh, Cedric S. Wesley, Michael W. Young

Biology and Genomics

Research output: Contribution to journal › Article › peer-review

Abstract

The cloning of double-time (db(t)) is reported. DOUBLETIME protein (DBT) is most closely related to human casein kinase Iε. dbt(S) and dbt(L) mutations, which alter period length of Drosophila circadian rhythms, produce single amino acid changes in conserved regions of the predicted kinase. dbt(P) mutants, which eliminate rhythms of per and tim expression and constitutively overproduce hypophosphorylated PER proteins, abolish most dbt expression. dbt mRNA appears to be expressed in the same cell types as are per and tim and shows no evident oscillation in wild-type heads. DBT is capable of binding to PER in vitro and in Drosophila cells, suggesting that a physical association of PER and DBT regulates PER phosphorylation and accumulation in vivo.

Original language	English (US)
Pages (from-to)	97-107
Number of pages	11
Journal	Cell
Volume	94
Issue number	1
DOIs	https://doi.org/10.1016/S0092-8674(00)81225-8
State	Published - Jul 10 1998

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/S0092-8674(00)81225-8

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title = "The Drosophila clock gene double-time encodes a protein closely related to human casein kinase Iε",

abstract = "The cloning of double-time (db(t)) is reported. DOUBLETIME protein (DBT) is most closely related to human casein kinase Iε. dbt(S) and dbt(L) mutations, which alter period length of Drosophila circadian rhythms, produce single amino acid changes in conserved regions of the predicted kinase. dbt(P) mutants, which eliminate rhythms of per and tim expression and constitutively overproduce hypophosphorylated PER proteins, abolish most dbt expression. dbt mRNA appears to be expressed in the same cell types as are per and tim and shows no evident oscillation in wild-type heads. DBT is capable of binding to PER in vitro and in Drosophila cells, suggesting that a physical association of PER and DBT regulates PER phosphorylation and accumulation in vivo.",

author = "Brian Kloss and Price, {Jeffrey L.} and Lino Saez and Justin Blau and Adrian Rothenfluh and Wesley, {Cedric S.} and Young, {Michael W.}",

note = "Funding Information: We thank Toby Lieber and Simon Kidd for advice and comments. We especially thank Simon Kidd for generously providing Drosophila embryonic and pupal cDNA libraries. We thank Karen Wager-Smith for important technical help in the early stages of this work. We thank James Briscoe for advice on in situ hybridization. J. B. was supported by the Human Frontier Science Program. A. R. was supported by a Beckman fellowship. This work was supported by NIH GM 54339 (M. W. Y), the NSF Science and Technology Center for Biological Timing (M. W. Y), and by NIH MH 56895 (J. L. P.). ",

year = "1998",

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doi = "10.1016/S0092-8674(00)81225-8",

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T1 - The Drosophila clock gene double-time encodes a protein closely related to human casein kinase Iε

AU - Kloss, Brian

AU - Price, Jeffrey L.

AU - Saez, Lino

AU - Blau, Justin

AU - Rothenfluh, Adrian

AU - Wesley, Cedric S.

AU - Young, Michael W.

N1 - Funding Information: We thank Toby Lieber and Simon Kidd for advice and comments. We especially thank Simon Kidd for generously providing Drosophila embryonic and pupal cDNA libraries. We thank Karen Wager-Smith for important technical help in the early stages of this work. We thank James Briscoe for advice on in situ hybridization. J. B. was supported by the Human Frontier Science Program. A. R. was supported by a Beckman fellowship. This work was supported by NIH GM 54339 (M. W. Y), the NSF Science and Technology Center for Biological Timing (M. W. Y), and by NIH MH 56895 (J. L. P.).

PY - 1998/7/10

Y1 - 1998/7/10

N2 - The cloning of double-time (db(t)) is reported. DOUBLETIME protein (DBT) is most closely related to human casein kinase Iε. dbt(S) and dbt(L) mutations, which alter period length of Drosophila circadian rhythms, produce single amino acid changes in conserved regions of the predicted kinase. dbt(P) mutants, which eliminate rhythms of per and tim expression and constitutively overproduce hypophosphorylated PER proteins, abolish most dbt expression. dbt mRNA appears to be expressed in the same cell types as are per and tim and shows no evident oscillation in wild-type heads. DBT is capable of binding to PER in vitro and in Drosophila cells, suggesting that a physical association of PER and DBT regulates PER phosphorylation and accumulation in vivo.

AB - The cloning of double-time (db(t)) is reported. DOUBLETIME protein (DBT) is most closely related to human casein kinase Iε. dbt(S) and dbt(L) mutations, which alter period length of Drosophila circadian rhythms, produce single amino acid changes in conserved regions of the predicted kinase. dbt(P) mutants, which eliminate rhythms of per and tim expression and constitutively overproduce hypophosphorylated PER proteins, abolish most dbt expression. dbt mRNA appears to be expressed in the same cell types as are per and tim and shows no evident oscillation in wild-type heads. DBT is capable of binding to PER in vitro and in Drosophila cells, suggesting that a physical association of PER and DBT regulates PER phosphorylation and accumulation in vivo.

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The Drosophila clock gene double-time encodes a protein closely related to human casein kinase Iε

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