The effect of a nanothickness coating on rough titanium substrate in the osteogenic properties of human bone cells

Camilla C.G. Moura, Maria A. Souza, Paula Dechichi, Darceny Zanetta-Barbosa, Cristina C. Teixeira, Paulo G. Coelho

Research output: Contribution to journalArticlepeer-review


This study evaluated the effect of a bioactive ceramic coating, in the nanothickness range, onto a moderately rough surface on the osteogenic behavior of human bone cells. The cells were harvested from the mandibular mental region and were cultured over Ti-6Al-4V disks of different surfaces: as-machined (M), alumina-blasted/acid etched (AB/AE), and aluminablasted/acid-etched + 300-500 nm thickness amorphous Ca- and P-based coating obtained by ion beam-assisted deposition (Nano). The culture was then evaluated regarding cell viability, adhesion, morphology, immunolocalization of osteopontin (OPN) and alkaline phosphatase (ALP). The results showed that the surface treatment did not interfere with cell viability. At 1 day, AB/AE and Nano showed higher adhesion than the M surface (p < 0.001). Higher adhesion was observed for the M than the Nano surface at 7 days (p < 0.005). The percentage of cells showing intracellular labeling for OPN at day 1 was significantly higher for the Nano compared to M surface (p < 0.03). The percentage of ALP intracellular labeling at 7 days was significantly higher for the AB/AE compared to the M surface (p < 0.0065); no differences were detected at 14 days. Our results suggest that the presence of a thin bioactive ceramic coating on a rough substrate did not favor the events related to in vitro osteogenesis.

Original languageEnglish (US)
Pages (from-to)103-111
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Issue number1
StatePublished - Jul 2010


  • Human bone cells
  • Nonothickness coating
  • Surface
  • Titanium

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys


Dive into the research topics of 'The effect of a nanothickness coating on rough titanium substrate in the osteogenic properties of human bone cells'. Together they form a unique fingerprint.

Cite this