TY - JOUR
T1 - The effect of brain-derived neurotrophic factor on periodontal furcation defects
AU - Jimbo, Ryo
AU - Tovar, Nick
AU - Janal, Malvin N.
AU - Mousa, Ramy
AU - Marin, Charles
AU - Yoo, Daniel
AU - Teixeira, Hellen S.
AU - Anchieta, Rodolfo B.
AU - Bonfante, Estevam A.
AU - Konishi, Akihiro
AU - Takeda, Katsuhiro
AU - Kurihara, Hidemi
AU - Coelho, Paulo G.
N1 - Funding Information:
This study was funded by Dentply IH, however, this does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.
PY - 2014/1/14
Y1 - 2014/1/14
N2 - This study aimed to observe the regenerative effect of brain-derived neurotrophic factor (BDNF) in a non-human primate furcation defect model. Class II furcation defects were created in the first and second molars of 8 non-human primates to simulate a clinical situation. The defect was filled with either, Group A: BDNF (500 μg/ml) in high-molecular weight-hyaluronic acid (HMW-HA), Group B: BDNF (50 μg/ml) in HMW-HA, Group C: HMW-HA acid only, Group D: empty defect, or Group E: BDNF (500 μg/ml) in saline. The healing status for all groups was observed at different time-points with micro computed tomography. The animals were euthanized after 11 weeks, and the tooth-bone specimens were subjected to histologic processing. The results showed that all groups seemed to successfully regenerate the alveolar buccal bone, however, only Group A regenerated the entire periodontal tissue, i.e., alveolar bone, cementum and periodontal ligament. It is suggested that the use of BDNF in combination with a scaffold such as the hyaluronic acid in periodontal furcation defects may be an effective treatment option.
AB - This study aimed to observe the regenerative effect of brain-derived neurotrophic factor (BDNF) in a non-human primate furcation defect model. Class II furcation defects were created in the first and second molars of 8 non-human primates to simulate a clinical situation. The defect was filled with either, Group A: BDNF (500 μg/ml) in high-molecular weight-hyaluronic acid (HMW-HA), Group B: BDNF (50 μg/ml) in HMW-HA, Group C: HMW-HA acid only, Group D: empty defect, or Group E: BDNF (500 μg/ml) in saline. The healing status for all groups was observed at different time-points with micro computed tomography. The animals were euthanized after 11 weeks, and the tooth-bone specimens were subjected to histologic processing. The results showed that all groups seemed to successfully regenerate the alveolar buccal bone, however, only Group A regenerated the entire periodontal tissue, i.e., alveolar bone, cementum and periodontal ligament. It is suggested that the use of BDNF in combination with a scaffold such as the hyaluronic acid in periodontal furcation defects may be an effective treatment option.
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U2 - 10.1371/journal.pone.0084845
DO - 10.1371/journal.pone.0084845
M3 - Article
C2 - 24454754
AN - SCOPUS:84897989993
SN - 1932-6203
VL - 9
JO - PloS one
JF - PloS one
IS - 1
M1 - e84845
ER -