TY - JOUR
T1 - The effect of skin grafting upon prolyl hydroxylase and hyaluronidase activities in mammalian wound repair
AU - Bertolami, Charles N.
AU - Donoff, R. Bruce
N1 - Funding Information:
’ This study was supported in part by grants from General Biomedical. Research Support Grant to the Massachusetts General Hospital and by Shriners Burns Center Burn Grant GM 21700 and National Institute of General Medical Science, ROl GM25850-I. z Clinical and Research Felloiv in Oral Surgery, Massachusetts General Hospital, Shriners’ Burns Institute. ’ Associate Professor of Oral Surgery, Harvard School of Dental Medicine, Associate Visiting Oral Surgeon, Massachusetts General Hospital and Shriners’ Burns Institute; to whom correspondence should be addressed.
PY - 1979/12
Y1 - 1979/12
N2 - In normally healing open wounds a five-fold increase in prolyl hydroxylase activity, an indicator of collagen synthesis, was observed between Days 5 and 15 which could be effectively suppressed by skin grafting. Decreased prolyl hydroxylase activities under grafts probably reflect decreased collagen synthesis. Collagen concentration (μg hydroxyproline/mg dry wt) increased in grafted granulation tissue while collagen per unit area decreased, suggesting a greater resorption of noncollagenous granulation tissue components. The removal of noncollagenous substances in open and grafted wound granulation tissue by the hyaluronidase described in the present study provides the first example of a functioning degradative system that could help to account for such collagen concentration increases.
AB - In normally healing open wounds a five-fold increase in prolyl hydroxylase activity, an indicator of collagen synthesis, was observed between Days 5 and 15 which could be effectively suppressed by skin grafting. Decreased prolyl hydroxylase activities under grafts probably reflect decreased collagen synthesis. Collagen concentration (μg hydroxyproline/mg dry wt) increased in grafted granulation tissue while collagen per unit area decreased, suggesting a greater resorption of noncollagenous granulation tissue components. The removal of noncollagenous substances in open and grafted wound granulation tissue by the hyaluronidase described in the present study provides the first example of a functioning degradative system that could help to account for such collagen concentration increases.
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U2 - 10.1016/0022-4804(79)90154-9
DO - 10.1016/0022-4804(79)90154-9
M3 - Article
C2 - 231150
AN - SCOPUS:0018629273
SN - 0022-4804
VL - 27
SP - 359
EP - 366
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 6
ER -