The Elusive Structure of Levocetirizine Dihydrochloride Determined by Electron Diffraction

Durga Prasad Karothu, Zainab Alhaddad, Christian R. Göb, Christian J. Schürmann, Robert Bücker, Panče Naumov

Research output: Contribution to journalArticlepeer-review


Levocetirizine is an orally administrated, second-generation antihistaminic active pharmaceutical ingredient that has been used to treat symptoms of allergy and long-term hives for over 25 years. Despite the wide use of this compound, its crystal structure has remained unknown. Here we report the application of 3D electron diffraction (3D ED)/Micro-crystal electron diffraction (MicroED) to determine the crystal structure of Levocetirizine dihydrochloride directly from crystalline powders that were extracted from commercially available tablets containing the compound. We also showcase the utility of dynamical refinement to unambiguously assign absolute configuration. The results highlight the immense potential of 3D ED/MicroED for structure elucidation of components of microcrystalline mixtures that obviates the need to grow large-size single crystals and the use of complementary analytical techniques, which could be important for identification as well as for primary structural characterization.

Original languageEnglish (US)
Article numbere202303761
JournalAngewandte Chemie - International Edition
Issue number26
StatePublished - Jun 26 2023


  • 3D Electron Diffraction
  • Active Pharmaceutical Ingredients
  • Crystallography
  • Levocetirizine Dihydrochloride
  • Microelectron Diffraction

ASJC Scopus subject areas

  • Catalysis
  • General Chemistry


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