The farnesyltransferase inhibitor FTI-277 suppresses the 24-kDa FGF2- induced radioresistance in HeLa cells expressing wild-type RAS

Elizabeth Cohen-Jonathan; Christine Toulas; Isabelle Ader; Sylvia Monteil; Cuider Allal; Jacques Bonnet; Andrew D. Hamilton; Said M. Sebti; Nicolas Daly-Schveitzer; Gilles Favre

doi:10.2307/3580225

The farnesyltransferase inhibitor FTI-277 suppresses the 24-kDa FGF2- induced radioresistance in HeLa cells expressing wild-type RAS

Elizabeth Cohen-Jonathan, Christine Toulas, Isabelle Ader, Sylvia Monteil, Cuider Allal, Jacques Bonnet, Andrew D. Hamilton, Said M. Sebti, Nicolas Daly-Schveitzer, Gilles Favre

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

In this paper, we describe the effect of the inhibitor of farnesyltransferase (FTI-277) on radioresistance induced by the 24-kDa isoform of FGF2 in human cells expressing wild-type RAS. Treatment with FTI- 277 (20 μM) for 48 h prior to irradiation led to a significant decrease in survival of radioresistant cells expressing the 24-kDa isoform (HeLa 3A) but had no effect on the survival of control cells (HeLa PINA). The radio- sensitizing effect of FTI-277 is accompanied by a stimulation of postmitotic cell death in HeLa 3A cells and by a reduction in G₂/M-phase arrest in both cell types. These results dearly demonstrate that at least one farnesylated protein is involved in the regulation of the radioresistance induced by the 24-kDa isoform of FGF2. Furthermore, the radiation-induced G₂/M-phase arrest is also under the control of farnesylated protein. This work also demonstrates that FTase inhibitors may be effective radio-sensitizers of certain human tumors with wild-type RAS.

Original language	English (US)
Pages (from-to)	404-411
Number of pages	8
Journal	Radiation Research
Volume	152
Issue number	4
DOIs	https://doi.org/10.2307/3580225
State	Published - Oct 1999

ASJC Scopus subject areas

Biophysics
Radiation
Radiology Nuclear Medicine and imaging

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The farnesyltransferase inhibitor FTI-277 suppresses the 24-kDa FGF2- induced radioresistance in HeLa cells expressing wild-type RAS. / Cohen-Jonathan, Elizabeth; Toulas, Christine; Ader, Isabelle; Monteil, Sylvia; Allal, Cuider; Bonnet, Jacques; Hamilton, Andrew D.; Sebti, Said M.; Daly-Schveitzer, Nicolas; Favre, Gilles.

In: Radiation Research, Vol. 152, No. 4, 10.1999, p. 404-411.

Research output: Contribution to journal › Article › peer-review

Cohen-Jonathan, Elizabeth ; Toulas, Christine ; Ader, Isabelle ; Monteil, Sylvia ; Allal, Cuider ; Bonnet, Jacques ; Hamilton, Andrew D. ; Sebti, Said M. ; Daly-Schveitzer, Nicolas ; Favre, Gilles. / The farnesyltransferase inhibitor FTI-277 suppresses the 24-kDa FGF2- induced radioresistance in HeLa cells expressing wild-type RAS. In: Radiation Research. 1999 ; Vol. 152, No. 4. pp. 404-411.

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title = "The farnesyltransferase inhibitor FTI-277 suppresses the 24-kDa FGF2- induced radioresistance in HeLa cells expressing wild-type RAS",

abstract = "In this paper, we describe the effect of the inhibitor of farnesyltransferase (FTI-277) on radioresistance induced by the 24-kDa isoform of FGF2 in human cells expressing wild-type RAS. Treatment with FTI- 277 (20 μM) for 48 h prior to irradiation led to a significant decrease in survival of radioresistant cells expressing the 24-kDa isoform (HeLa 3A) but had no effect on the survival of control cells (HeLa PINA). The radio- sensitizing effect of FTI-277 is accompanied by a stimulation of postmitotic cell death in HeLa 3A cells and by a reduction in G2/M-phase arrest in both cell types. These results dearly demonstrate that at least one farnesylated protein is involved in the regulation of the radioresistance induced by the 24-kDa isoform of FGF2. Furthermore, the radiation-induced G2/M-phase arrest is also under the control of farnesylated protein. This work also demonstrates that FTase inhibitors may be effective radio-sensitizers of certain human tumors with wild-type RAS.",

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AU - Allal, Cuider

AU - Bonnet, Jacques

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AU - Favre, Gilles

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The farnesyltransferase inhibitor FTI-277 suppresses the 24-kDa FGF2- induced radioresistance in HeLa cells expressing wild-type RAS

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