The filamentous phage (Ff) as vectors for recombinant DNA- a review

Norton D. Zinder, Jef D. Boeke

    Research output: Contribution to journalArticle

    Abstract

    Derivatives of filamentous phage, f1, fd, and M 13, useful as cloning vectors are listed, and procedures for their use are reviewed. Methods for growing phage, preparing single- and double-stranded DNA, and cloning are given in the "cook-book" form. These procedures minimize the practical problem often associated with filamentous-phage cloning, i.e., deletion of inserts.

    Original languageEnglish (US)
    Pages (from-to)1-10
    Number of pages10
    JournalGene
    Volume19
    Issue number1
    DOIs
    StatePublished - 1982

    Keywords

    • DNA preparation method
    • Single-stranded cloning vehicles
    • deletion

    ASJC Scopus subject areas

    • Genetics

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