TY - JOUR
T1 - The GATA Factor HANABA TARANU Is Required to Position the Proembryo Boundary in the Early Arabidopsis Embryo
AU - Nawy, Tal
AU - Bayer, Martin
AU - Mravec, Jozef
AU - Friml, Jiří
AU - Birnbaum, Kenneth D.
AU - Lukowitz, Wolfgang
N1 - Funding Information:
We are indebted to Chris Somerville for generous support early in the project and to Bastiaan Bargmann for assistance with protoplasting and cell sorting. Elliot Meyerowitz provided the han-2 allele, Hugo Hofhuis the pPLT1:CFP reporter, and Adrienne Roeder the pML1:H2B-YFP reporter. We would like to thank Azadeh Akhavan and Mary Galli for providing experimental help, and Bastiaan Bargmann, Stewart Gilmor, Sangho Jeong, and Dolf Weijers for helpful discussions and critical comments on the manuscript. This work was supported by the National Science Foundation (grant IOB-0842284 to W.L.), National Institutes of Health (grant R01 GM078279 to K.D.B), the Cold Spring Harbor Laboratory Association (postdoctoral fellowship to T.N.), and the Deutsche Forschungsgemeinschaft (postdoctoral fellowship to M.B.).
PY - 2010/7
Y1 - 2010/7
N2 - Division of the Arabidopsis zygote defines two fundamentally different developmental domains, the proembryo and suspensor. The resulting boundary separates domain-specific gene expression, and a signal originating from the proembryo instructs the suspensor to generate the root stem cell niche. While root induction is known to require the phytohormone auxin and the Auxin Response Factor MONOPTEROS, it has remained largely elusive how the two domains involved in this process are initially specified. Here, we show that the GATA factor HANABA TARANU (HAN) is required to position the inductive proembryo boundary. Mutations in HAN cause a coordinated apical shift of gene expression patterns, revealing that HAN regulates transcription in the basal proembryo. Key auxin transporters are affected as early as the 8 cell stage, resulting in apical redistribution of auxin. Remarkably, han embryos eventually organize a root independent of MONOPTEROS and the suspensor around a new boundary marked by the auxin maximum.
AB - Division of the Arabidopsis zygote defines two fundamentally different developmental domains, the proembryo and suspensor. The resulting boundary separates domain-specific gene expression, and a signal originating from the proembryo instructs the suspensor to generate the root stem cell niche. While root induction is known to require the phytohormone auxin and the Auxin Response Factor MONOPTEROS, it has remained largely elusive how the two domains involved in this process are initially specified. Here, we show that the GATA factor HANABA TARANU (HAN) is required to position the inductive proembryo boundary. Mutations in HAN cause a coordinated apical shift of gene expression patterns, revealing that HAN regulates transcription in the basal proembryo. Key auxin transporters are affected as early as the 8 cell stage, resulting in apical redistribution of auxin. Remarkably, han embryos eventually organize a root independent of MONOPTEROS and the suspensor around a new boundary marked by the auxin maximum.
KW - Devbio
UR - http://www.scopus.com/inward/record.url?scp=77954921614&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77954921614&partnerID=8YFLogxK
U2 - 10.1016/j.devcel.2010.06.004
DO - 10.1016/j.devcel.2010.06.004
M3 - Article
C2 - 20643354
AN - SCOPUS:77954921614
SN - 1534-5807
VL - 19
SP - 103
EP - 113
JO - Developmental Cell
JF - Developmental Cell
IS - 1
ER -