The genome and phenome of the green alga chloroidium sp. UTEX 3007 reveal adaptive traits for desert acclimatization

David R. Nelson; Basel Khraiwesh; Weiqi Fu; Saleh Alseekh; Ashish Jaiswal; Amphun Chaiboonchoe; Khaled M. Hazzouri; Matthew J. O’Connor; Glenn L. Butterfoss; Nizar Drou; Jillian D. Rowe; Jamil Harb; Alisdair R. Fernie; Kristin C. Gunsalus; Kourosh Salehi-Ashtiani

doi:10.7554/eLife.25783

The genome and phenome of the green alga chloroidium sp. UTEX 3007 reveal adaptive traits for desert acclimatization

David R. Nelson, Basel Khraiwesh, Weiqi Fu, Saleh Alseekh, Ashish Jaiswal, Amphun Chaiboonchoe, Khaled M. Hazzouri, Matthew J. O’Connor, Glenn L. Butterfoss, Nizar Drou, Jillian D. Rowe, Jamil Harb, Alisdair R. Fernie, Kristin C. Gunsalus, Kourosh Salehi-Ashtiani

Research output: Contribution to journal › Article › peer-review

Abstract

To investigate the phenomic and genomic traits that allow green algae to survive in deserts, we characterized a ubiquitous species, Chloroidium sp. UTEX 3007, which we isolated from multiple locations in the United Arab Emirates (UAE). Metabolomic analyses of Chloroidium sp. UTEX 3007 indicated that the alga accumulates a broad range of carbon sources, including several desiccation tolerance-promoting sugars and unusually large stores of palmitate. Growth assays revealed capacities to grow in salinities from zero to 60 g/L and to grow heterotrophically on >40 distinct carbon sources. Assembly and annotation of genomic reads yielded a 52.5 Mbp genome with 8153 functionally annotated genes. Comparison with other sequenced green algae revealed unique protein families involved in osmotic stress tolerance and saccharide metabolism that support phenomic studies. Our results reveal the robust and flexible biology utilized by a green alga to successfully inhabit a desert coastline.

Original language	English (US)
Article number	e25783
Journal	eLife
Volume	6
DOIs	https://doi.org/10.7554/eLife.25783
State	Published - Jun 17 2017

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

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10.7554/eLife.25783

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Nelson, D. R., Khraiwesh, B., Fu, W., Alseekh, S., Jaiswal, A., Chaiboonchoe, A., Hazzouri, K. M., O’Connor, M. J., Butterfoss, G. L., Drou, N., Rowe, J. D., Harb, J., Fernie, A. R., Gunsalus, K. C., & Salehi-Ashtiani, K. (2017). The genome and phenome of the green alga chloroidium sp. UTEX 3007 reveal adaptive traits for desert acclimatization. eLife, 6, Article e25783. https://doi.org/10.7554/eLife.25783

Nelson, DR, Khraiwesh, B, Fu, W, Alseekh, S, Jaiswal, A, Chaiboonchoe, A, Hazzouri, KM, O’Connor, MJ, Butterfoss, GL, Drou, N, Rowe, JD, Harb, J, Fernie, AR, Gunsalus, KC & Salehi-Ashtiani, K 2017, 'The genome and phenome of the green alga chloroidium sp. UTEX 3007 reveal adaptive traits for desert acclimatization', eLife, vol. 6, e25783. https://doi.org/10.7554/eLife.25783

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abstract = "To investigate the phenomic and genomic traits that allow green algae to survive in deserts, we characterized a ubiquitous species, Chloroidium sp. UTEX 3007, which we isolated from multiple locations in the United Arab Emirates (UAE). Metabolomic analyses of Chloroidium sp. UTEX 3007 indicated that the alga accumulates a broad range of carbon sources, including several desiccation tolerance-promoting sugars and unusually large stores of palmitate. Growth assays revealed capacities to grow in salinities from zero to 60 g/L and to grow heterotrophically on >40 distinct carbon sources. Assembly and annotation of genomic reads yielded a 52.5 Mbp genome with 8153 functionally annotated genes. Comparison with other sequenced green algae revealed unique protein families involved in osmotic stress tolerance and saccharide metabolism that support phenomic studies. Our results reveal the robust and flexible biology utilized by a green alga to successfully inhabit a desert coastline.",

author = "Nelson, {David R.} and Basel Khraiwesh and Weiqi Fu and Saleh Alseekh and Ashish Jaiswal and Amphun Chaiboonchoe and Hazzouri, {Khaled M.} and O{\textquoteright}Connor, {Matthew J.} and Butterfoss, {Glenn L.} and Nizar Drou and Rowe, {Jillian D.} and Jamil Harb and Fernie, {Alisdair R.} and Gunsalus, {Kristin C.} and Kourosh Salehi-Ashtiani",

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AU - Nelson, David R.

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AU - Jaiswal, Ashish

AU - Chaiboonchoe, Amphun

AU - Hazzouri, Khaled M.

AU - O’Connor, Matthew J.

AU - Butterfoss, Glenn L.

AU - Drou, Nizar

AU - Rowe, Jillian D.

AU - Harb, Jamil

AU - Fernie, Alisdair R.

AU - Gunsalus, Kristin C.

AU - Salehi-Ashtiani, Kourosh

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The genome and phenome of the green alga chloroidium sp. UTEX 3007 reveal adaptive traits for desert acclimatization

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