The influence of diabetes on gingival crevicular fluid β-glucuronidase and interleukin-8

Steven P. Engebretson, Faranak Vossughi, Judith Hey-Hadavi, Gülnur Emingil, John T. Grbic

Research output: Contribution to journalArticlepeer-review


Objectives: Polymorphonuclear neutrophil (PMN) dysfunction is associated with diabetes. We examined the gingival crevicular fluid (GCF) β-glucuronidase (BG) and interleukin-8 (IL-8) levels of periodontitis patients with and without type 2 diabetes mellitus (DM). Material and methods: Forty five adults with type 2 DM and 32 adults without DM, both with chronic periodontitis were enrolled. GCF was collected from eight posterior sites in each quadrant, and periodontal parameters were recorded. GCF was assayed for IL-8 by ELISA and BG by a flourometric assay. Results: GCF IL-8 was positively correlated with probing depth (PD), and GCF BG but not clinical attachment level (CAL), bleeding on probing (BOP), or plaque index (PI). In contrast, GCF BG was strongly correlated with each of the clinical measures of periodontal disease. Subjects with DM significantly lower levels of both BG (73.0±44.8 versus 121.9±84.6 pg/sample; p=0.002) and IL-8 (32.1±33.1 versus 90.8±83.2 pg/sample; p<0.0001) even after adjustments for age, gender, PD, CAL, BOP, and PI. Neither BG nor IL-8 was correlated with HbA1c levels in subjects with DM. Conclusion: These data suggest that an inadequate local response by PMN, partially explained by an altered chemokine gradient, may contribute to periodontal disease in patients with type 2 DM.

Original languageEnglish (US)
Pages (from-to)784-790
Number of pages7
JournalJournal of clinical periodontology
Issue number11
StatePublished - Nov 2006


  • Adult
  • Diabetes mellitus
  • Gingival crevicular fluid
  • HbA1c
  • Interleukin-8
  • PMN
  • Periodontitis
  • β-glucuronidase

ASJC Scopus subject areas

  • Periodontics


Dive into the research topics of 'The influence of diabetes on gingival crevicular fluid β-glucuronidase and interleukin-8'. Together they form a unique fingerprint.

Cite this