TY - JOUR
T1 - The influence of terminal 3′,5′ phosphates on conformations of dApdA
AU - Broyde, S.
AU - Hingerty, B.
N1 - Funding Information:
ACKNOWLEDGEMENT Grateful acknowledgement is made to the U.S. Public Health Service for support of this work under NIH Grant 5R01GM24482-02 and to the Division of Biomedical and Environmental Research, U.S. Department of Energy, under contract W-7405-eng-26 with the Union Carbide Corporation.
Funding Information:
•Research sponsored jointly by the U.S. Public Health Service under NIH Grant 5R01GM24482-02 and the Division of Biomedical and Environmental Research, U.S. Departnent of Energy, under contract W-7405-eng-26 with the Union Carbide Corporation.
PY - 1979
Y1 - 1979
N2 - Addition of 3′ and 5′ terminal phosphates to dApdA causes a decrease in conformational flexibility. pdApdAp has much fewer conformers with energies below 2.5 kcal./mole than dApdA. The A, B and Watson-Crick (34) helices are the most preferred forms. Other important conformations are in the trans domain of ψ. Thus, flexibility in ψ, as well as in ω and ω, and in the sugar pucker is indicated. The transformation from the B helix to the Watson-Crick helix follows a low energy path. This is significant since Watson-Crick conformations may be important for intercalation into nucleic acid polymers (40-42) above the dimer level. The B helix is preferred over the A form in these larger DNA subunits.
AB - Addition of 3′ and 5′ terminal phosphates to dApdA causes a decrease in conformational flexibility. pdApdAp has much fewer conformers with energies below 2.5 kcal./mole than dApdA. The A, B and Watson-Crick (34) helices are the most preferred forms. Other important conformations are in the trans domain of ψ. Thus, flexibility in ψ, as well as in ω and ω, and in the sugar pucker is indicated. The transformation from the B helix to the Watson-Crick helix follows a low energy path. This is significant since Watson-Crick conformations may be important for intercalation into nucleic acid polymers (40-42) above the dimer level. The B helix is preferred over the A form in these larger DNA subunits.
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U2 - 10.1093/nar/6.6.2165
DO - 10.1093/nar/6.6.2165
M3 - Article
C2 - 461185
AN - SCOPUS:0018337846
SN - 0305-1048
VL - 6
SP - 2165
EP - 2178
JO - Nucleic acids research
JF - Nucleic acids research
IS - 6
ER -