The isolation and sequence analysis of vasoactive intestinal peptide from a ganglioneuroblastoma

N. W. Bunnett; J. R. Reeve; R. Dimaline; J. E. Shively; D. Hawke; J. H. Walsh

doi:10.1210/jcem-59-6-1133

The isolation and sequence analysis of vasoactive intestinal peptide from a ganglioneuroblastoma

N. W. Bunnett, J. R. Reeve, R. Dimaline, J. E. Shively, D. Hawke, J. H. Walsh

Molecular Pathobiology

Research output: Contribution to journal › Article › peer-review

Abstract

A ganglioneuroblastoma was excised at surgery from a 1-yr-old girl with severe watery diarrhea. The tumor, weighing 1 g, was extracted in trifluoracetic acid and contained 8.3 nmol immunoreactive vasoactive intestinal peptide. The peptide was isolated by affinity chromatography and high pressure liquid chromatography and was found to be identical to porcine vasoactive intestinal peptide by amino acid analysis and microsequence analysis.

Original language	English (US)
Pages (from-to)	1133-1137
Number of pages	5
Journal	Journal of Clinical Endocrinology and Metabolism
Volume	59
Issue number	6
DOIs	https://doi.org/10.1210/jcem-59-6-1133
State	Published - Dec 1984

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Endocrinology
Clinical Biochemistry
Biochemistry, medical

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10.1210/jcem-59-6-1133

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AU - Reeve, J. R.

AU - Dimaline, R.

AU - Shively, J. E.

AU - Hawke, D.

AU - Walsh, J. H.

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AB - A ganglioneuroblastoma was excised at surgery from a 1-yr-old girl with severe watery diarrhea. The tumor, weighing 1 g, was extracted in trifluoracetic acid and contained 8.3 nmol immunoreactive vasoactive intestinal peptide. The peptide was isolated by affinity chromatography and high pressure liquid chromatography and was found to be identical to porcine vasoactive intestinal peptide by amino acid analysis and microsequence analysis.

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The isolation and sequence analysis of vasoactive intestinal peptide from a ganglioneuroblastoma

Abstract

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