The label-free unambiguous detection and symbolic display of single nucleotide polymorphisms on DNA origami

Hari K.K. Subramanian, Banani Chakraborty, Ruojie Sha, Nadrian C. Seeman

Research output: Contribution to journalArticlepeer-review

Abstract

Single nucleotide polymorphisms (SNPs) are the most common genetic variation in the human genome. Kinetic methods based on branch migration have proved successful for detecting SNPs because a mispair inhibits the progress of branch migration in the direction of the mispair. We have combined the effectiveness of kinetic methods with atomic force microscopy of DNA origami patterns to produce a direct visual readout of the target nucleotide contained in the probe sequence. The origami contains graphical representations of the four nucleotide alphabetic characters, A, T, G and C, and the symbol containing the test nucleotide identity vanishes in the presence of the probe. The system also works with pairs of probes, corresponding to heterozygous diploid genomes.

Original languageEnglish (US)
Pages (from-to)910-913
Number of pages4
JournalNano Letters
Volume11
Issue number2
DOIs
StatePublished - Feb 9 2011

Keywords

  • DNA branch migration
  • DNA origami
  • Single nucleotide polymorphism detection
  • atomic force microscopy
  • direct readout of SNPs
  • photo-cleavable linkers

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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