@article{c50b7b0ddf944d8ab5bc92103909aa02,
title = "The nuclear activity of the actin-binding Moesin protein is necessary for gene expression in Drosophila",
abstract = "Ezrin–Radixin–Moesin (ERM) proteins play an essential role in the cytoplasm by cross-linking actin filaments with plasma membrane proteins. Research has identified the nuclear localization of ERMs, as well as the involvement of a single Drosophila ERM protein, Moesin, in nuclear mRNA exports. However, the question of how important the nuclear activity of ERM proteins are for the life of an organism has so far not been explored. Here, we present the first attempt to reveal the in vivo relevance of nuclear localization of Moesin in Drosophila. With the help of a nuclear export signal, we decreased the amount of Moesin in the nuclei of the animals. Furthermore, we observed various developmental defects, demonstrating the importance of ERM function in the nucleus for the first time. Transcriptome analysis of the mutant flies revealed that the lack of nuclear Moesin function leads to expression changes in nearly 700 genes, among them heat-shock genes. This result together with additional findings revealed that in Drosophila the expression of protein chaperones requires the nuclear functions of Moesin. Database: GEO accession number: GSE155778.",
keywords = "Drosophila, Hsp, Moesin, nucleus, transcription",
author = "Csaba Bajusz and Ildik{\'o} Krist{\'o} and Csilla Abonyi and Tom{\'a}{\v s} Venit and Viktor Vedelek and Tam{\'a}s Luk{\'a}csovich and Attila Farkas and P{\'e}ter Bork{\'u}ti and Zolt{\'a}n Kov{\'a}cs and Izabella Bajusz and Annam{\'a}ria Marton and Csaba Vizler and Zolt{\'a}n Lipinszki and Rita Sinka and Piergiorgio Percipalle and P{\'e}ter Vilmos",
note = "Funding Information: We thank Henrik Gyurkovics (BRC, Szeged) for helpful discussions, Ildik{\'o} Velkeyn{\'e} Krausz and Anik{\'o} Berente for technical assistance. Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) were used in this study. This work was supported by the Ministry for National Economy of Hungary grants GINOP-2.3.2-15-2016-00001 and GINOP-2.3.2-15-2016-00032 (to PV and ZL), the Hungarian National Research, Development and Innovation Office—NKFIH grants PD127968 (to IK), NKFIH-871-3/2020 (to PV), and K132155 (to RS and VV), the Hungarian Academy of Sciences grant LP2017-7/2017 (to ZL), and partly supported by an NYU Abu Dhabi Research Enhancement Fund and a grant from the Swedish Cancer Society (Cancerfonden) (to PP). Funding Information: We thank Henrik Gyurkovics (BRC, Szeged) for helpful discussions, Ildik{\'o} Velkeyn{\'e} Krausz and Anik{\'o} Berente for technical assistance. Stocks obtained from the Bloomington Drosophila Stock Center (NIH P40OD018537) were used in this study. This work was supported by the Ministry for National Economy of Hungary grants GINOP‐2.3.2‐15‐2016‐00001 and GINOP‐2.3.2‐15‐2016‐00032 (to PV and ZL), the Hungarian National Research, Development and Innovation Office—NKFIH grants PD127968 (to IK), NKFIH‐871‐3/2020 (to PV), and K132155 (to RS and VV), the Hungarian Academy of Sciences grant LP2017‐7/2017 (to ZL), and partly supported by an NYU Abu Dhabi Research Enhancement Fund and a grant from the Swedish Cancer Society (Cancerfonden) (to PP). Publisher Copyright: {\textcopyright} 2021 The Authors. The FEBS Journal published by John Wiley & Ltd on behalf of Federation of European Biochemical Societies.",
year = "2021",
month = aug,
doi = "10.1111/febs.15779",
language = "English (US)",
volume = "288",
pages = "4812--4832",
journal = "FEBS Journal",
issn = "1742-464X",
publisher = "Wiley-Blackwell",
number = "16",
}