@article{60599cd91d554a7fae6988aaf807219d,
title = "The organization and development of cortical interneuron presynaptic circuits are area specific",
abstract = "Parvalbumin and somatostatin inhibitory interneurons gate information flow in discrete cortical areas that compute sensory and cognitive functions. Despite the considerable differences between areas, individual interneuron subtypes are genetically invariant and are thought to form canonical circuits regardless of which area they are embedded in. Here, we investigate whether this is achieved through selective and systematic variations in their afferent connectivity during development. To this end, we examined the development of their inputs within distinct cortical areas. We find that interneuron afferents show little evidence of being globally stereotyped. Rather, each subtype displays characteristic regional connectivity and distinct developmental dynamics by which this connectivity is achieved. Moreover, afferents dynamically regulated during development are disrupted by early sensory deprivation and in a model of fragile X syndrome. These data provide a comprehensive map of interneuron afferents across cortical areas and reveal the logic by which these circuits are established during development.",
keywords = "ALM, GABAergic interneurons, cortical areas, development, fragile X syndrome, monosynaptic rabies tracing, sensory cortex, thalamocortical input",
author = "Gabrielle Pouchelon and Deepanjali Dwivedi and Yannick Bollmann and Agba, {Chimuanya K.} and Qing Xu and Mirow, {Andrea M.C.} and Sehyun Kim and Yanjie Qiu and Elaine Sevier and Ritola, {Kimberly D.} and Rosa Cossart and Gord Fishell",
note = "Funding Information: This work was supported by an EMBO Long-Term fellowship, early and advanced Swiss Foundation postdoctoral fellowships, a Hearst foundation grant (to G.P.), and grants from the National Institutes of Health (NIH), MH071679 , NS08297 , NS074972 , MH095147 , as well as support from the Simons Foundation (SFARI) (to GF). Funding Information: We thank Nusrath Yusuf and Marian Fernandez-Otero for their support all along the project and Justin McMahon for the plasmid submission to Addgene. This work was supported by an EMBO Long-Term fellowship, early and advanced Swiss Foundation postdoctoral fellowships, a Hearst foundation grant (to G.P.), and grants from the National Institutes of Health (NIH), MH071679, NS08297, NS074972, MH095147, as well as support from the Simons Foundation (SFARI) (to GF). G.P. and G.F. conceived the project and wrote the manuscript. G.P. and C.K.A. carried out injection experiments. Y.Q. S.K. and G.P. processed brains for quantification. D.D. performed and analyzed physiological intrinsic properties. Y.B. designed and performed the automated analysis with the help of G.P. G.P. and A.M.C.M. manually quantified connectivity. R.C. contributed to the design of automated analysis. E.F. contributed to the design of rabies injection. K.D.R. provided guidance on N2cRV and AAV use as well as produced nuclear-tdtomato N2cRV. Gord Fishell is a founder of Regel Therapeutics, which has no competing interests with the present manuscript. We worked to ensure sex balance in the selection of non-human subjects. One or more of the authors of this paper self-identifies as an underrepresented ethnic minority in science. One or more of the authors of this paper self-identifies as a member of the LGBTQ+ community. Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = nov,
day = "9",
doi = "10.1016/j.celrep.2021.109993",
language = "English (US)",
volume = "37",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "6",
}