TY - JOUR
T1 - The plant hormone ethylene restricts Arabidopsis growth via the epidermis
AU - Vaseva, Irina Ivanova
AU - Qudeimat, Enas
AU - Potuschak, Thomas
AU - Du, Yunlong
AU - Genschik, Pascal
AU - Vandenbussche, Filip
AU - Van Der Straeten, Dominique
N1 - Funding Information:
ACKNOWLEDGMENTS. We thank Corina Codreanu for helping with vector constructs and measurements of root cell lengths, Magdalena Baltova and Mathias De Mesmaeker for preliminary experiments regarding cell elongation in root tissues, Justyna Nocon and Bram Van Compernolle for basic cloning steps, and Joke Belza for verifying the ethylene concentration in the ethylene exposure experiment. We also thank Malcolm Bennett for helpful suggestions and critical reading of the manuscript. D.V.D.S. thanks Jiri Friml and Eva Benkova for constructive discussions on auxin-related experiments. D.V.D.S. received financial support from the Research Foundation Flanders (Projects G.0298.09 and G.0656.13N) and Ghent University (BOF-BAS 01B02112). T.P. and P.G. were supported by Laboratoire d’Excellence (Grant ANR-10-LABX-0036_NETRNA).
Funding Information:
We thank Corina Codreanu for helping with vector constructs and measurements of root cell lengths, Magdalena Baltova and Mathias De Mesmaeker for preliminary experiments regarding cell elongation in root tissues, Justyna Nocon and Bram Van Compernolle for basic cloning steps, and Joke Belza for verifying the ethylene concentration in the ethylene exposure experiment. We also thank Malcolm Bennett for helpful suggestions and critical reading of the manuscript. D.V.D.S. thanks Jiri Friml and Eva Benkova for constructive discussions on auxin-related experiments. D.V.D.S. received financial support from the Research Foundation Flanders (Projects G.0298.09 and G.0656.13N) and Ghent University (BOF-BAS 01B02112). T.P. and P.G. were supported by Laboratoire d’Excellence (Grant ANR-10-LABX-0036_NETRNA).
Publisher Copyright:
© 2018 National Academy of Sciences. All Rights Reserved.
PY - 2018/4/24
Y1 - 2018/4/24
N2 - The gaseous hormone ethylene plays a key role in plant growth and development, and it is a major regulator of stress responses. It inhibits vegetative growth by restricting cell elongation, mainly through cross-talk with auxins. However, it remains unknown whether ethylene controls growth throughout all plant tissues or whether its signaling is confined to specific cell types. We employed a targeted expression approach to map the tissue site(s) of ethylene growth regulation. The ubiquitin E3 ligase complex containing Skp1, Cullin1, and the F-box protein EBF1 or EBF2 (SCFEBF1/2) target the degradation of EIN3, the master transcription factor in ethylene signaling. We coupled EBF1 and EBF2 to a number of cell type-specific promoters. Using phenotypic assays for ethylene response and mutant complementation, we revealed that the epidermis is the main site of ethylene action controlling plant growth in both roots and shoots. Suppression of ethylene signaling in the epidermis of the constitutive ethylene signaling mutant ctr1-1 was sufficient to rescue the mutant phenotype, pointing to the epidermis as a key cell type required for ethylene-mediated growth inhibition.
AB - The gaseous hormone ethylene plays a key role in plant growth and development, and it is a major regulator of stress responses. It inhibits vegetative growth by restricting cell elongation, mainly through cross-talk with auxins. However, it remains unknown whether ethylene controls growth throughout all plant tissues or whether its signaling is confined to specific cell types. We employed a targeted expression approach to map the tissue site(s) of ethylene growth regulation. The ubiquitin E3 ligase complex containing Skp1, Cullin1, and the F-box protein EBF1 or EBF2 (SCFEBF1/2) target the degradation of EIN3, the master transcription factor in ethylene signaling. We coupled EBF1 and EBF2 to a number of cell type-specific promoters. Using phenotypic assays for ethylene response and mutant complementation, we revealed that the epidermis is the main site of ethylene action controlling plant growth in both roots and shoots. Suppression of ethylene signaling in the epidermis of the constitutive ethylene signaling mutant ctr1-1 was sufficient to rescue the mutant phenotype, pointing to the epidermis as a key cell type required for ethylene-mediated growth inhibition.
KW - Arabidopsis
KW - Auxin
KW - EIN3 binding F-box factor EBF
KW - Ethylene
KW - Root/shoot
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U2 - 10.1073/pnas.1717649115
DO - 10.1073/pnas.1717649115
M3 - Article
C2 - 29643073
AN - SCOPUS:85045933676
SN - 0027-8424
VL - 115
SP - E4130-E4139
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 17
ER -