The poly(rU) coil: A minimum‐energy model that matches experimental observations

Brian E. Hingerty; Suse B. Broyde; Wilma K. Olson

doi:10.1002/bip.360210612

The poly(rU) coil: A minimum‐energy model that matches experimental observations

Brian E. Hingerty, Suse B. Broyde, Wilma K. Olson

Biology and Genomics

Research output: Contribution to journal › Article › peer-review

Abstract

A model of the randomly coiling form of poly(rU) based on minimum‐energy conformers of UpU is described. The blend of conformers is chosen to fit the CC rotational populations derived in nmr studies of UpU and poly(rU) and to match the experimental unperturbed dimensions of the poly(rU) chain. In addition, estimates of loop closure based on the model are comparable to the sizes of loops most frequently seen in model oligonucleotides. Approximately 60% of the conformers constituting the model are characterized by stacked, extended C2′‐endo ω′ωψ = tg⁻g⁺ rotations. The remainder of the chain is described by equal numbers of C3′‐endo A (ω′ωψ = g⁻g⁻g⁺) and Watson‐Crick (ω′ωψ = g⁻tt) helical arrangements.

Original language	English (US)
Pages (from-to)	1167-1188
Number of pages	22
Journal	Biopolymers
Volume	21
Issue number	6
DOIs	https://doi.org/10.1002/bip.360210612
State	Published - Jun 1982

ASJC Scopus subject areas

Biophysics
Biochemistry
Biomaterials
Organic Chemistry

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10.1002/bip.360210612

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abstract = "A model of the randomly coiling form of poly(rU) based on minimum‐energy conformers of UpU is described. The blend of conformers is chosen to fit the CC rotational populations derived in nmr studies of UpU and poly(rU) and to match the experimental unperturbed dimensions of the poly(rU) chain. In addition, estimates of loop closure based on the model are comparable to the sizes of loops most frequently seen in model oligonucleotides. Approximately 60% of the conformers constituting the model are characterized by stacked, extended C2′‐endo ω′ωψ = tg−g+ rotations. The remainder of the chain is described by equal numbers of C3′‐endo A (ω′ωψ = g−g−g+) and Watson‐Crick (ω′ωψ = g−tt) helical arrangements.",

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The poly(rU) coil: A minimum‐energy model that matches experimental observations

Abstract

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