The potential of stromal cell-derived factor-1 delivery using a collagen membrane for bone regeneration

Tadahiro Takayama, Jisen Dai, Keita Tachi, Ryutaro Shohara, Hironori Kasai, Kentaro Imamura, Seiichi Yamano

Research output: Contribution to journalArticle

Abstract

Stromal cell-derived factor-1 (SDF-1) is a cytokine that is important in stem and progenitor cell recruitment in tissue repair after injury. Regenerative procedures using collagen membranes (CMs) are presently well established in periodontal and implant dentistry. The objective of this study is to test the subsequent effects of the released SDF-1 from a CM on bone regeneration compared to platelet-derived growth factor (PDGF) in vitro and in vivo. For in vitro studies, cell proliferation, alkaline phosphatase activity, and osteoblastic differentiation marker genes were assessed after MC3T3-E1 mouse preosteoblasts were cultured with CMs containing factors. In vivo effects were investigated by placement of CMs containing SDF-1 or PDGF using a rat mandibular bone defect model. At 4 weeks after the surgery, the new bone formation was measured using micro-computed tomography (μCT) and histological analysis. The results of in vitro studies revealed that CM delivery of SDF-1 significantly induced cell proliferation, ALP activity, and gene expression of all osteogenic markers compared to the CM alone or control, similar to PDGF. Quantitative and qualitative μCT analysis for volume of new bone formation and the percentage of new bone area showed that SDF-1-treated groups significantly increased and accelerated bone regeneration compared to control and CM alone. The enhancement of bone formation in SDF-1-treated animals was dose-dependent and with levels similar to those measured with PDGF. These results suggest that a CM with SDF-1 may be a great candidate for growth factor delivery that could be a substitute for PDGF in clinical procedures where bone regeneration is necessary.

Original languageEnglish (US)
Pages (from-to)1049-1061
Number of pages13
JournalJournal of Biomaterials Applications
Volume31
Issue number7
DOIs
StatePublished - Feb 1 2017

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Collagen
Bone
Membranes
Bronchiolo-Alveolar Adenocarcinoma
Platelets
Cell proliferation
Blood Flow Velocity
Dentistry
Phosphatases
Gene expression
Surgery
Tomography
Rats
Animals
Repair
Genes
Tissue
Defects
Library Catalogs
Cyclic AMP Receptor Protein

Keywords

  • Bone regeneration
  • collagen membrane
  • osteogenesis
  • PDGF
  • SDF-1

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

Cite this

The potential of stromal cell-derived factor-1 delivery using a collagen membrane for bone regeneration. / Takayama, Tadahiro; Dai, Jisen; Tachi, Keita; Shohara, Ryutaro; Kasai, Hironori; Imamura, Kentaro; Yamano, Seiichi.

In: Journal of Biomaterials Applications, Vol. 31, No. 7, 01.02.2017, p. 1049-1061.

Research output: Contribution to journalArticle

Takayama T, Dai J, Tachi K, Shohara R, Kasai H, Imamura K et al. The potential of stromal cell-derived factor-1 delivery using a collagen membrane for bone regeneration. Journal of Biomaterials Applications. 2017 Feb 1;31(7):1049-1061. Available from, DOI: 10.1177/0885328216686727

Takayama, Tadahiro; Dai, Jisen; Tachi, Keita; Shohara, Ryutaro; Kasai, Hironori; Imamura, Kentaro; Yamano, Seiichi / The potential of stromal cell-derived factor-1 delivery using a collagen membrane for bone regeneration.

In: Journal of Biomaterials Applications, Vol. 31, No. 7, 01.02.2017, p. 1049-1061.

Research output: Contribution to journalArticle

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