@article{c7712a56645c47a8993cd7823ef0f8fa,
title = "The Requirement of Nkx2-1 in the Temporal Specification of Cortical Interneuron Subtypes",
abstract = "Previous work has demonstrated that the character of mouse cortical interneuron subtypes can be directly related to their embryonic temporal and spatial origins. The relationship between embryonic origin and the character of mature interneurons is likely reflected by the developmental expression of genes that direct cell fate. However, a thorough understanding of the early genetic events that specify subtype identity has been hampered by the perinatal lethality resulting from the loss of genes implicated in the determination of cortical interneurons. Here, we employ a conditional loss-of-function approach to demonstrate that the transcription factor Nkx2-1 is required for the proper specification of specific interneuron subtypes. Removal of this gene at distinct neurogenic time points results in a switch in the subtypes of neurons observed at more mature ages. Our strategy reveals a causal link between the embryonic genetic specification by Nkx2-1 in progenitors and the functional attributes of their neuronal progeny in the mature nervous system.",
keywords = "DEVBIO, SIGNALING",
author = "Butt, {Simon J.B.} and Sousa, {Vitor H.} and Fuccillo, {Marc V.} and Jens Hjerling-Leffler and Goichi Miyoshi and Shioko Kimura and Gord Fishell",
note = "Funding Information: We wish to thank Dr. Hirohide Takebayashi for providing the Olig2 CreER{\texttrademark} mouse. cDNA in situ probe templates were kindly provided by J. Ericson (Nkx6-2), A. Joyner (Cre, Gli1, Nkx2-1, Shh), V. Pachnis (Lhx6), J. Rubenstein (Dlx2), and M. Studer (CoupTF-II). The polyclonal rabbit anti-Lhx6 antibody was a kind gift from V. Pachnis. We would also like to thank Lihong Yin and Jiali Deng for excellent technical help. S.J.B.B. is a recipient of a Human Frontier Science Program Organization Long-Term Fellowship; J.H.-L. is a recipient of a postdoctoral fellowship from the Swedish Brain Foundation (Hj{\"a}rnfonden); G.M. is supported by a grant from the Japan Society for the Promotion of Science. Research in the Fishell laboratory is supported by National Institutes of Health (National Institute of Mental Health and National Institute of Neurological Disorders and Stroke Grants R01MH068469 and R01NS039007). S.K. is supported by the Intramural Research Program of the National Cancer Institute, Center for Cancer Research. ",
year = "2008",
month = sep,
day = "11",
doi = "10.1016/j.neuron.2008.07.031",
language = "English (US)",
volume = "59",
pages = "722--732",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "5",
}