The roles of annexins and types II and X collagen in matrix vesicle-mediated mineralization of growth plate cartilage

Thorsten Kirsch, Gerald Harrison, Ellis E. Golub, Hyun Duck Nah

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Annexins II, V, and VI are major components of matrix vesicles (MV), i.e. particles that have the critical role of initiating the mineralization process in skeletal tissues. Furthermore, types II and X collagen are associated with MV, and these interactions mediated by annexin V stimulate Ca2+ uptake and mineralization of MV. However, the exact roles of annexin II, V, and VI and the interaction between annexin V and types II and X collagen in MV function and initiation of mineralization are not well understood. In this study, we demonstrate that annexin II, V, or VI mediate Ca2+ influx into phosphatidylserine (PS)-enriched liposomes, liposomes containing lipids extracted from authentic MV, and intact authentic MV. The annexin Ca2+ channel blocker, K-201, not only inhibited Ca2+ influx into fura-2-loaded PSenriched liposomes mediated by annex-in II, V, or VI, but also inhibited Ca2+ uptake by authentic MV. Types II and X collagen only bound to liposomes in the presence of annexin V but not in the presence of annexin II or VI. Binding of these collagens to annexin V stimulated its Ca2+ channel activities, leading to an increased Ca2+ influx into the liposomes. These findings indicate that the formation of annexin II, V, and VI Ca2+ channels in MV together with stimulation of annexin V channel activity by collagen (types II and X) binding can explain how MV are able to rapidly take up Ca2+ and initiate the formation of the first crystal phase.

    Original languageEnglish (US)
    Pages (from-to)35577-35583
    Number of pages7
    JournalJournal of Biological Chemistry
    Volume275
    Issue number45
    DOIs
    StatePublished - Nov 10 2000

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Fingerprint Dive into the research topics of 'The roles of annexins and types II and X collagen in matrix vesicle-mediated mineralization of growth plate cartilage'. Together they form a unique fingerprint.

    Cite this