The saccharomyces cerevisiae SCRaMbLE system and genome minimization

Jessica Dymond, Jef Boeke

Research output: Contribution to journalArticlepeer-review


We have recently reported the first partially synthetic eukaryotic genome. Saccharomyces cerevisiae chromosomes synIXR and semi-synVIL are fully synthetic versions of the right arm of chromosome IX and the telomeric segment of the left arm of chromosome VI, respectively, and represent the beginning of the synthetic yeast genome project, Sc2.0, that progressively replaces native yeast DNA with synthetic sequences. We have designed synthetic chromosome sequences according to principles specifying a wild-type phenotype, highly stable genome, and maintenance of genetic flexibility. Although other synthetic genome projects exist, the Sc2.0 approach is unique in that we have implemented design specifications predicted to generate a wild-type phenotype until induction of "SCRaMbLE," an inducible evolution system that generates significant genetic diversity. Here we further explore the significance of Sc2.0 and show how SCRaMbLE can serve as a genome minimization tool.

Original languageEnglish (US)
Pages (from-to)168-171
Number of pages4
JournalBioengineered Bugs
Issue number3
StatePublished - Jul 2012


  • Genome minimization
  • Sc2.0
  • Synthetic genome
  • Yeast
  • synVIL

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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