TY - JOUR
T1 - The temporal and spatial origins of cortical interneurons predict their physiological subtype
AU - Butt, Simon J.B.
AU - Fuccillo, Marc
AU - Nery, Susana
AU - Noctor, Steven
AU - Kriegstein, Arnold
AU - Corbin, Joshua G.
AU - Fishell, Gord
N1 - Funding Information:
We are grateful to members of the Fishell lab for comments on the manuscript. We would specifically like to thank Goichi Miyoshi for advice on the BrdU experiments. We are also indebted to Dr. Bernardo Rudy and Ethan Goldberg for discussion, technical advice, and the generous supply of Kv3.1 and Kv3.2 antibodies. We would like to thank Dr. Okabe for the gift of his CAG gfp transgenic mouse. The following people provided cDNA probes and antibodies: J. Rubenstein (Dlx2, Lhx6), S. Kimura (Nkx2.1), P. Trainor (Cux2) and (ErbB4), B. Rudy (Kv3.1), S. Pleasure (SFRP2), and J. Kohtz (pan-Dlx). Finally, we would like to thank Yuan-Yuan Huang and Rebecca Wolsky for excellent technical assistance with transplantations and sectioning. S.B. is a recipient of the Human Frontiers Science Program long-term fellowship. Research in the Fishell lab is sponsored by the National Institutes of Health.
PY - 2005/11/23
Y1 - 2005/11/23
N2 - Interneurons of the cerebral cortex represent a heterogeneous population of cells with important roles in network function. At present, little is known about how these neurons are specified in the developing telencephalon. To explore whether this diversity is established in the early progenitor populations, we conducted in utero fate-mapping of the mouse medial and caudal ganglionic eminences (MGE and CGE, respectively), from which most cortical interneurons arise. Mature interneuron subtypes were assessed by electrophysiological and immunological analysis, as well as by morphological reconstruction. At E13.5, the MGE gives rise to fast-spiking (FS) interneurons, whereas the CGE generates predominantly regular-spiking interneurons (RSNP). Later at E15.5, the CGE produces RSNP classes distinct from those generated from the E13.5 CGE. Thus, we provide evidence that the spatial and temporal origin of interneuron precursors in the developing telencephalic eminences predicts the intrinsic physiological properties of mature interneurons.
AB - Interneurons of the cerebral cortex represent a heterogeneous population of cells with important roles in network function. At present, little is known about how these neurons are specified in the developing telencephalon. To explore whether this diversity is established in the early progenitor populations, we conducted in utero fate-mapping of the mouse medial and caudal ganglionic eminences (MGE and CGE, respectively), from which most cortical interneurons arise. Mature interneuron subtypes were assessed by electrophysiological and immunological analysis, as well as by morphological reconstruction. At E13.5, the MGE gives rise to fast-spiking (FS) interneurons, whereas the CGE generates predominantly regular-spiking interneurons (RSNP). Later at E15.5, the CGE produces RSNP classes distinct from those generated from the E13.5 CGE. Thus, we provide evidence that the spatial and temporal origin of interneuron precursors in the developing telencephalic eminences predicts the intrinsic physiological properties of mature interneurons.
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U2 - 10.1016/j.neuron.2005.09.034
DO - 10.1016/j.neuron.2005.09.034
M3 - Article
C2 - 16301176
AN - SCOPUS:27844432222
SN - 0896-6273
VL - 48
SP - 591
EP - 604
JO - Neuron
JF - Neuron
IS - 4
ER -