Abstract
In male mice, the transcription factors STRA8 and MEISON initiate meiosis I. We report that STRA8/MEISON activates the transcription factors A-MYB and TCFL5, which together reprogram gene expression after spermatogonia enter into meiosis. TCFL5 promotes the transcription of genes required for meiosis, mRNA turnover, miR-34/449 production, meiotic exit, and spermiogenesis. This transcriptional architecture is conserved in rhesus macaque, suggesting TCFL5 plays a central role in meiosis and spermiogenesis in placental mammals. Tcfl5em1/em1 mutants are sterile, and spermatogenesis arrests at the mid- or late-pachytene stage of meiosis. Moreover, Tcfl5+/em1 mutants produce fewer motile sperm.
Original language | English (US) |
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Pages (from-to) | 183-196 |
Number of pages | 14 |
Journal | Reproduction |
Volume | 165 |
Issue number | 2 |
DOIs | |
State | Published - Feb 2023 |
ASJC Scopus subject areas
- Reproductive Medicine
- Embryology
- Endocrinology
- Obstetrics and Gynecology
- Cell Biology