The transcription factor TCFL5 responds to A-MYB to elaborate the male meiotic program in mice

Katharine Cecchini, Adriano Biasini, Tianxiong Yu, Martin Säflund, Haiwei Mou, Amena Arif, Atiyeh Eghbali, Cansu Colpan, Ildar Gainetdinov, Dirk G. de Rooij, Zhiping Weng, Phillip D. Zamore, Deniz M. Özata

Research output: Contribution to journalArticlepeer-review

Abstract

In male mice, the transcription factors STRA8 and MEISON initiate meiosis I. We report that STRA8/MEISON activates the transcription factors A-MYB and TCFL5, which together reprogram gene expression after spermatogonia enter into meiosis. TCFL5 promotes the transcription of genes required for meiosis, mRNA turnover, miR-34/449 production, meiotic exit, and spermiogenesis. This transcriptional architecture is conserved in rhesus macaque, suggesting TCFL5 plays a central role in meiosis and spermiogenesis in placental mammals. Tcfl5em1/em1 mutants are sterile, and spermatogenesis arrests at the mid- or late-pachytene stage of meiosis. Moreover, Tcfl5+/em1 mutants produce fewer motile sperm.

Original languageEnglish (US)
Pages (from-to)183-196
Number of pages14
JournalReproduction
Volume165
Issue number2
DOIs
StatePublished - Feb 2023

ASJC Scopus subject areas

  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Obstetrics and Gynecology
  • Cell Biology

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