@article{5f52e8cf52bf419a9dface57c98726bd,
title = "The twin spot generator for differential Drosophila lineage analysis",
abstract = "In Drosophila melanogaster, widely used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the twin spot generator (TSG) technique, which through mitotic recombination generates green and red twin spots that are detectable after the first cell division as single cells. We propose wide applications of TSG to lineage and genetic mosaic studies.",
author = "Ruth Griffin and Anne Sustar and Marianne Bonvin and Richard Binari and {del Valle Rodriguez}, Alberto and Hohl, {Amber M.} and Bateman, {Jack R.} and Christians Villalta and Elleard Heffern and Didier Grunwald and Chris Bakal and Claude Desplan and Gerold Schubiger and Wu, {C. Ting} and Norbert Perrimon",
note = "Funding Information: We thank J. Zirin and M. Packard for confocal expertise, P. Bradley for molecular biology advice, F. Karch (University of Geneva) for providing flies carrying integrase and T.S. Griffin for contributing the technique acronym. This work was supported by grants from the US National Institutes of Health (1 RO1 GM61936, C.-t.W.; RO1 GM058282, G.S.; and RO1 GM084947, N.P.) and a Ruth L. Kirschstein National Research Service award (1 F32 GM67460, J.R.B.); the US National Science Foundation (A.M.H.); the Swiss National Science Foundation (PBBE33-121069, M.B.) and the Leukemia and Lymphoma Society (C.B.).",
year = "2009",
doi = "10.1038/nmeth.1349",
language = "English (US)",
volume = "6",
pages = "600--602",
journal = "Nature methods",
issn = "1548-7091",
publisher = "Public Library of Science",
number = "8",
}