The whispering gallery mode biosensor: Label-free detection from virus to single protein

S. Holler, V. R. Dantham, D. Keng, V. Kolchenko, S. Arnold, Brigid Mulroe, M. Paspaley-Grbavac

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The whispering gallery mode (WGM) biosensor is a micro-optical platform capable of sensitive label-free detection of biological particles. Described by the reactive sensing principle (RSP), this analytic formulation quantifies the response of the system to the adsorption of bioparticles. Guided by the RSP, the WGM biosensor enabling from detection of virus (e.g., Human Papillomavirus, HPV) to the ultimate goal of single protein detection. The latter was derived from insights into the RSP, which resulted in the development of a hybrid plasmonic WGM biosensor, which has recently demonstrated detection of individual protein cancer markers. Enhancements from bound gold nanoparticles provide the sensitivity to detect single protein molecules (66 kDa) with good signal-to-noise (S/N > 10), and project that detection of proteins as small as 5 kDa.

Original languageEnglish (US)
Title of host publicationBiosensing and Nanomedicine VII
EditorsMassoud H. Agahi, Hooman Mohseni, Manijeh Razeghi
PublisherSPIE
ISBN (Electronic)1628411937, 9781628411935
DOIs
StatePublished - 2014
EventBiosensing and Nanomedicine VII - San Diego, United States
Duration: Aug 17 2014 → …

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume9166
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Other

OtherBiosensing and Nanomedicine VII
Country/TerritoryUnited States
CitySan Diego
Period8/17/14 → …

Keywords

  • Whispering gallery mode
  • biosensing
  • microcavity
  • photonics
  • plasmonics
  • reactive sensing principle
  • resonance
  • single protein

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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