Therapeutic effect of localized vibration on alveolar bone of osteoporotic rats

Mani Alikhani, Mona Alikhani, Sarah Alansari, Abdullah Almansour, Mohammad A. Hamidaddin, Edmund Khoo, Jose A. Lopez, Jeanne M. Nervina, Joo Y. Nho, Serafim M. Oliveira, Chinapa Sangsuwon, Cristina Teixeira

Research output: Contribution to journalArticle

Abstract

Objectives Vibration, in the form of high frequency acceleration (HFA), stimulates alveolar bone formation under physiologic conditions and during healing after dental extractions. It is not known if HFA has an anabolic effect on osteoporotic alveolar bone. Our objective is to determine if HFA has a regenerative effect on osteoporotic alveolar bone. Methods and materials Adult female Sprague-Dawley rats were divided into five groups: 1) Ovariectomized Group (OVX), 2) Sham-OVX Group that received surgery without ovariectomy, 3) OVX-HFA Group that was ovariectomized and treated daily with HFA, 4) OVX+Static Force Group that was ovariectomized and received the same force as HFA, but without vibration, and 5) Control Group that did not receive any treatment. All animals were fed a low mineral diet for 3 months. Osteoporosis was confirmed by micro-CT of the fifth lumbar vertebra and femoral head. HFA was applied to the maxillary first molar for 5 minutes/day for 28 and 56 days. Maxillae were collected for micro-CT, histology, fluorescent microscopy, protein and RNA analysis, and three-point bending mechanical testing. Results Micro-CT analysis revealed significant alveolar bone osteoporosis in the OVX group. Vibration restored the quality and quantity of alveolar bone to levels similar to the Sham-OVX group. Animals exposed to HFA demonstrated higher osteoblast activity and lower osteoclast activity. Osteogenic transcription factors (RUNX2, Foxo1, Osterix and Wnt signaling factors) were upregulated following vibration, while RANKL/RANK and Sclerostin were downregulated. HFA did not affect serum TRAcP-5b or CTx-1 levels. The osteogenic effect was highest at the point of HFA application and extended along the hemimaxillae this effect did not cross to the contra-lateral side. Conclusions Local application of vibration generated gradients of increased anabolic metabolism and decreased catabolic metabolism in alveolar bone of osteoporotic rats. Our findings suggest that HFA could be a predictable treatment for diminished alveolar bone levels in osteoporosis patients.

Original languageEnglish (US)
Article numbere0211004
JournalPLoS One
Volume14
Issue number1
DOIs
StatePublished - Jan 1 2019

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Therapeutic Uses
Vibration
vibration
Rats
Bone
bones
Bone and Bones
therapeutics
rats
micro-computed tomography
osteoporosis
Osteoporosis
Metabolism
Animals
Wnt Proteins
Anabolic Agents
Tooth Extraction
Lumbar Vertebrae
osteoclasts
Histology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Alikhani, M., Alikhani, M., Alansari, S., Almansour, A., Hamidaddin, M. A., Khoo, E., ... Teixeira, C. (2019). Therapeutic effect of localized vibration on alveolar bone of osteoporotic rats. PLoS One, 14(1), [e0211004]. https://doi.org/10.1371/journal.pone.0211004

Therapeutic effect of localized vibration on alveolar bone of osteoporotic rats. / Alikhani, Mani; Alikhani, Mona; Alansari, Sarah; Almansour, Abdullah; Hamidaddin, Mohammad A.; Khoo, Edmund; Lopez, Jose A.; Nervina, Jeanne M.; Nho, Joo Y.; Oliveira, Serafim M.; Sangsuwon, Chinapa; Teixeira, Cristina.

In: PLoS One, Vol. 14, No. 1, e0211004, 01.01.2019.

Research output: Contribution to journalArticle

Alikhani, M, Alikhani, M, Alansari, S, Almansour, A, Hamidaddin, MA, Khoo, E, Lopez, JA, Nervina, JM, Nho, JY, Oliveira, SM, Sangsuwon, C & Teixeira, C 2019, 'Therapeutic effect of localized vibration on alveolar bone of osteoporotic rats', PLoS One, vol. 14, no. 1, e0211004. https://doi.org/10.1371/journal.pone.0211004
Alikhani M, Alikhani M, Alansari S, Almansour A, Hamidaddin MA, Khoo E et al. Therapeutic effect of localized vibration on alveolar bone of osteoporotic rats. PLoS One. 2019 Jan 1;14(1). e0211004. https://doi.org/10.1371/journal.pone.0211004
Alikhani, Mani ; Alikhani, Mona ; Alansari, Sarah ; Almansour, Abdullah ; Hamidaddin, Mohammad A. ; Khoo, Edmund ; Lopez, Jose A. ; Nervina, Jeanne M. ; Nho, Joo Y. ; Oliveira, Serafim M. ; Sangsuwon, Chinapa ; Teixeira, Cristina. / Therapeutic effect of localized vibration on alveolar bone of osteoporotic rats. In: PLoS One. 2019 ; Vol. 14, No. 1.
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abstract = "Objectives Vibration, in the form of high frequency acceleration (HFA), stimulates alveolar bone formation under physiologic conditions and during healing after dental extractions. It is not known if HFA has an anabolic effect on osteoporotic alveolar bone. Our objective is to determine if HFA has a regenerative effect on osteoporotic alveolar bone. Methods and materials Adult female Sprague-Dawley rats were divided into five groups: 1) Ovariectomized Group (OVX), 2) Sham-OVX Group that received surgery without ovariectomy, 3) OVX-HFA Group that was ovariectomized and treated daily with HFA, 4) OVX+Static Force Group that was ovariectomized and received the same force as HFA, but without vibration, and 5) Control Group that did not receive any treatment. All animals were fed a low mineral diet for 3 months. Osteoporosis was confirmed by micro-CT of the fifth lumbar vertebra and femoral head. HFA was applied to the maxillary first molar for 5 minutes/day for 28 and 56 days. Maxillae were collected for micro-CT, histology, fluorescent microscopy, protein and RNA analysis, and three-point bending mechanical testing. Results Micro-CT analysis revealed significant alveolar bone osteoporosis in the OVX group. Vibration restored the quality and quantity of alveolar bone to levels similar to the Sham-OVX group. Animals exposed to HFA demonstrated higher osteoblast activity and lower osteoclast activity. Osteogenic transcription factors (RUNX2, Foxo1, Osterix and Wnt signaling factors) were upregulated following vibration, while RANKL/RANK and Sclerostin were downregulated. HFA did not affect serum TRAcP-5b or CTx-1 levels. The osteogenic effect was highest at the point of HFA application and extended along the hemimaxillae this effect did not cross to the contra-lateral side. Conclusions Local application of vibration generated gradients of increased anabolic metabolism and decreased catabolic metabolism in alveolar bone of osteoporotic rats. Our findings suggest that HFA could be a predictable treatment for diminished alveolar bone levels in osteoporosis patients.",
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AU - Khoo, Edmund

AU - Lopez, Jose A.

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N2 - Objectives Vibration, in the form of high frequency acceleration (HFA), stimulates alveolar bone formation under physiologic conditions and during healing after dental extractions. It is not known if HFA has an anabolic effect on osteoporotic alveolar bone. Our objective is to determine if HFA has a regenerative effect on osteoporotic alveolar bone. Methods and materials Adult female Sprague-Dawley rats were divided into five groups: 1) Ovariectomized Group (OVX), 2) Sham-OVX Group that received surgery without ovariectomy, 3) OVX-HFA Group that was ovariectomized and treated daily with HFA, 4) OVX+Static Force Group that was ovariectomized and received the same force as HFA, but without vibration, and 5) Control Group that did not receive any treatment. All animals were fed a low mineral diet for 3 months. Osteoporosis was confirmed by micro-CT of the fifth lumbar vertebra and femoral head. HFA was applied to the maxillary first molar for 5 minutes/day for 28 and 56 days. Maxillae were collected for micro-CT, histology, fluorescent microscopy, protein and RNA analysis, and three-point bending mechanical testing. Results Micro-CT analysis revealed significant alveolar bone osteoporosis in the OVX group. Vibration restored the quality and quantity of alveolar bone to levels similar to the Sham-OVX group. Animals exposed to HFA demonstrated higher osteoblast activity and lower osteoclast activity. Osteogenic transcription factors (RUNX2, Foxo1, Osterix and Wnt signaling factors) were upregulated following vibration, while RANKL/RANK and Sclerostin were downregulated. HFA did not affect serum TRAcP-5b or CTx-1 levels. The osteogenic effect was highest at the point of HFA application and extended along the hemimaxillae this effect did not cross to the contra-lateral side. Conclusions Local application of vibration generated gradients of increased anabolic metabolism and decreased catabolic metabolism in alveolar bone of osteoporotic rats. Our findings suggest that HFA could be a predictable treatment for diminished alveolar bone levels in osteoporosis patients.

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