Thermosiphon-based PCR reactor: Experiment and modeling

Zongyuan Chen, Shizhi Qian, William R. Abrams, Daniel Malamud, Haim H. Bau

Research output: Contribution to journalArticlepeer-review

Abstract

A self-actuated, flow-cycling polymerase chain reaction (PCR) reactor that takes advantage of buoyancy forces to continuously circulate reagents in a closed loop through various thermal zones has been constructed, tested, and modeled. The heating required for the PCR is advantageously used to induce fluid motion without the need for a pump. Flow velocities on the order of millimeters per second are readily attainable. In our preliminary proto-type, we measured a cross-sectionally averaged velocity of 2.5 mm/s and a cycle time of 104 s. The flow velocity is nearly independent of the loop's length, making the device readily scalable. Successful amplifications of 700- and 305-bp fragments of Bacillus cereus genomic DNA have been demonstrated. Since the device does not require any moving parts, it is particularly suitable for miniature systems.

Original languageEnglish (US)
Pages (from-to)3707-3715
Number of pages9
JournalAnalytical chemistry
Volume76
Issue number13
DOIs
StatePublished - Jul 1 2004

ASJC Scopus subject areas

  • Analytical Chemistry

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