@article{90439ef3fa3f4d698ebf9401dd73d1d8,
title = "Thermus thermophilus Argonaute Functions in the Completion of DNA Replication",
abstract = "In many eukaryotes, Argonaute proteins, guided by short RNA sequences, defend cells against transposons and viruses. In the eubacterium Thermus thermophilus, the DNA-guided Argonaute TtAgo defends against transformation by DNA plasmids. Here, we report that TtAgo also participates in DNA replication. In vivo, TtAgo binds 15- to 18-nt DNA guides derived from the chromosomal region where replication terminates and associates with proteins known to act in DNA replication. When gyrase, the sole T. thermophilus type II topoisomerase, is inhibited, TtAgo allows the bacterium to finish replicating its circular genome. In contrast, loss of gyrase and TtAgo activity slows growth and produces long sausage-like filaments in which the individual bacteria are linked by DNA. Finally, wild-type T. thermophilus outcompetes an otherwise isogenic strain lacking TtAgo. We propose that the primary role of TtAgo is to help T. thermophilus disentangle the catenated circular chromosomes generated by DNA replication.",
keywords = "Argonaute, DNA replication, RNA silencing, Thermus thermophilus, TtAgo, decatenation, gyrase, pAGO, terminus of replication, topoisomerase",
author = "Jolly, {Samson M.} and Ildar Gainetdinov and Karina Jouravleva and Han Zhang and Lara Strittmatter and Bailey, {Shannon M.} and Hendricks, {Gregory M.} and Avantika Dhabaria and Beatrix Ueberheide and Zamore, {Phillip D.}",
note = "Funding Information: We thank Christina Baer for help with imaging, Daniel Tom and Douglas Matthews (Leica) for assistance with STED microscopy, Nicholas Rhind for critical analysis and guidance, Ahmet Ozturk for help with Circos software, and members of the Zamore laboratory for discussions and comments on the manuscript. This work was supported in part by NIGMS grant R37GM062862 to P.D.Z. and National Center for Research Resources grants S10OD025113 , S10RR021043 , and SI0OD021580 to G.M.H. Funding Information: We thank Christina Baer for help with imaging, Daniel Tom and Douglas Matthews (Leica) for assistance with STED microscopy, Nicholas Rhind for critical analysis and guidance, Ahmet Ozturk for help with Circos software, and members of the Zamore laboratory for discussions and comments on the manuscript. This work was supported in part by NIGMS grant R37GM062862 to P.D.Z. and National Center for Research Resources grants S10OD025113, S10RR021043, and SI0OD021580 to G.M.H. Conceptualization, P.D.Z. and S.M.J.; Methodology, S.M.J. I.G. K.J. G.M.H. and A.D.; Experiments, S.M.J. I.G. H.Z. K.J. L.S. A.D. and S.M.B.; Resources, G.M.H. and B.U.; Formal Analyses, I.G. S.M.J. K.J. and A.D.; Writing – Original Draft, S.M.J.; Writing – Review & Editing, P.D.Z. and S.M.J.; Supervision and Funding Acquisition, P.D.Z. P.D.Z. S.M.J. and H.Z. have submitted a patent application regarding novel uses of TtAgo (PCT/US2016/025724). Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
month = sep,
day = "17",
doi = "10.1016/j.cell.2020.07.036",
language = "English (US)",
volume = "182",
pages = "1545--1559.e18",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "6",
}