Three-dimensional optical tomographic brain imaging in small animals, part 2: Unilateral carotid occlusion

A. Y. Bluestone, M. Stewart, B. Lei, I. S. Kass, J. Lasker, G. S. Abdoulaev, A. H. Hielscher

    Research output: Contribution to journalArticlepeer-review

    Abstract

    This is the second part of a two-part study that explores the feasibility of 3-D, volumetric brain imaging in small animals by optical tomographic techniques. In part 1, we demonstrated the ability to visualize global hemodynamic changes in the rat head in response to elevated levels of CO 2 using a continuous-wave instrument and model-based iterative image reconstruction (MOBIIR) algorithm. Now we focus on lateralized, monohemispherically localized hemodynamic effects generated by unilateral common carotid artery (CCA) occlusion. This illustrates the capability of our optical tomographic system to localize and distinguish hemodynamic responses in different parts of the brain. Unilateral carotid occlusions are performed in ten rodents under two experimental conditions. In the first set of experiments the normal systemic blood pressure is lowered to 50 mmHg, and on unilateral carotid occlusion, we observe an ipsilateral monohemispheric global decrease in blood volume and oxygenation. This finding is consistent with the known physiologic response to cerebral ischemia. In a second set of experiments designed to observe the spatial-temporal dynamics of CCA occlusion at normotensive blood pressure, more complex phenomena are observed. We find three different types of responses, which can be categorized as compensation, overcompensation, and noncompensation.

    Original languageEnglish (US)
    Pages (from-to)1063-1073
    Number of pages11
    JournalJournal of biomedical optics
    Volume9
    Issue number5
    DOIs
    StatePublished - Sep 2004

    Keywords

    • Brain imaging
    • Carotid occlusion
    • Optical tomography
    • Rodents
    • Small animal model

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Biomaterials
    • Atomic and Molecular Physics, and Optics
    • Biomedical Engineering

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