TY - JOUR
T1 - THz characterization of DNA four-way junction and its components
AU - Slvovska, Maryna I.
AU - Seeman, Nadrian C.
AU - Sha, Ruojie
AU - Globus, Tatiana R.
AU - Khromova, Tatyana B.
AU - Dorofeeva, Tatiana S.
N1 - Funding Information:
Manuscript received January 3, 2010; revised March 15, 2010; accepted April 1, 2010. Date of publication May 3, 2010; date of current version September 9, 2010. This work was supported in part by the U.S. Army Research Office (ARO) under Funding W911NF-08-1-0138 and Funding HDTRA-08-1-0038, in part by the U.S. Army N61 c under Contract DASC01-01-C0009, and in part by the ARO under Grant W911FF-08-C-0057, via subcontract from Pegasus Corporation. The review of this paper was arranged by Associate Editor A. A. Balandin.
PY - 2010/9
Y1 - 2010/9
N2 - The four-way DNA junction that is a structural building block for DNA self-assemblies has been widely studied by diffraction methods and other physical techniques. However, terahertz (THz) spectroscopic characterization, which can reveal specific absorption fingerprints from different DNA structures and sequences, has not been reported yet. In this study, we present the spectral features of the four-way nucleic acid junction, its single- and double-stranded components, and their stoichiometric mixtures in the 10-25 cm-1 frequency range. We demonstrated that sub-THz vibrational spectroscopy can be used for sensing and characterizing the nanosize biomolecular architectures with welldefined composition and sequence. The results show that we can distinguish between the spectral features for all materials examined. Samples with amounts of material as low as 400 ng can be characterized. Thus, the resolved vibrational spectroscopy in the low-THz range can be used as a powerful tool to characterize the DNA junction structures and its components, as well as to monitor the J1 junction formation process.
AB - The four-way DNA junction that is a structural building block for DNA self-assemblies has been widely studied by diffraction methods and other physical techniques. However, terahertz (THz) spectroscopic characterization, which can reveal specific absorption fingerprints from different DNA structures and sequences, has not been reported yet. In this study, we present the spectral features of the four-way nucleic acid junction, its single- and double-stranded components, and their stoichiometric mixtures in the 10-25 cm-1 frequency range. We demonstrated that sub-THz vibrational spectroscopy can be used for sensing and characterizing the nanosize biomolecular architectures with welldefined composition and sequence. The results show that we can distinguish between the spectral features for all materials examined. Samples with amounts of material as low as 400 ng can be characterized. Thus, the resolved vibrational spectroscopy in the low-THz range can be used as a powerful tool to characterize the DNA junction structures and its components, as well as to monitor the J1 junction formation process.
KW - DNA self-assembly
KW - Four-way junction
KW - Terahertz (THz) spectroscopy
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U2 - 10.1109/TNANO.2010.2049498
DO - 10.1109/TNANO.2010.2049498
M3 - Article
AN - SCOPUS:77956637223
SN - 1536-125X
VL - 9
SP - 610
EP - 617
JO - IEEE Transactions on Nanotechnology
JF - IEEE Transactions on Nanotechnology
IS - 5
ER -