TY - JOUR
T1 - Time lapse microscopy of temperature control during self-assembly of 3D DNA crystals
AU - Conn, Fiona W.
AU - Jong, Michael Alexander
AU - Tan, Andre
AU - Tseng, Robert
AU - Park, Eunice
AU - Ohayon, Yoel P.
AU - Sha, Ruojie
AU - Mao, Chengde
AU - Seeman, Nadrian C.
N1 - Funding Information:
This research has been supported by the following grants to Nadrian C. Seeman: USA NSF EFRI-1332411, and CCF-1526650 from the NSF, MURI W911NF-11-1-0024 from ARO, MURI N000140911118 from ONR, DE-SC0007991 from DOE for DNA synthesis and partial salary support, and grant GBMF3849 from the Gordon and Betty Moore Foundation.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/10/15
Y1 - 2017/10/15
N2 - DNA nanostructures are created by exploiting the high fidelity base-pairing interactions of double-stranded branched DNA molecules. These structures present a convenient medium for the self-assembly of macroscopic 3D crystals. In some self-assemblies in this system, crystals can be formed by lowering the temperature, and they can be dissolved by raising it. The ability to monitor the formation and melting of these crystals yields information that can be used to monitor crystal formation and growth. Here, we describe the development of an inexpensive tool that enables direct observation of the crystal growth process as a function of both time and temperature. Using the hanging-drop crystallization of the well-characterized 2-turn DNA tensegrity triangle motif for our model system, its response to temperature has been characterized visually.
AB - DNA nanostructures are created by exploiting the high fidelity base-pairing interactions of double-stranded branched DNA molecules. These structures present a convenient medium for the self-assembly of macroscopic 3D crystals. In some self-assemblies in this system, crystals can be formed by lowering the temperature, and they can be dissolved by raising it. The ability to monitor the formation and melting of these crystals yields information that can be used to monitor crystal formation and growth. Here, we describe the development of an inexpensive tool that enables direct observation of the crystal growth process as a function of both time and temperature. Using the hanging-drop crystallization of the well-characterized 2-turn DNA tensegrity triangle motif for our model system, its response to temperature has been characterized visually.
KW - A2. Growth from solution
KW - B1. Cyanine
KW - B3. Microscope cameras
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U2 - 10.1016/j.jcrysgro.2017.07.019
DO - 10.1016/j.jcrysgro.2017.07.019
M3 - Article
AN - SCOPUS:85026806171
SN - 0022-0248
VL - 476
SP - 1
EP - 5
JO - Journal of Crystal Growth
JF - Journal of Crystal Growth
ER -