Time lapse microscopy of temperature control during self-assembly of 3D DNA crystals

Fiona W. Conn, Michael Alexander Jong, Andre Tan, Robert Tseng, Eunice Park, Yoel P. Ohayon, Ruojie Sha, Chengde Mao, Nadrian C. Seeman

Research output: Contribution to journalArticlepeer-review


DNA nanostructures are created by exploiting the high fidelity base-pairing interactions of double-stranded branched DNA molecules. These structures present a convenient medium for the self-assembly of macroscopic 3D crystals. In some self-assemblies in this system, crystals can be formed by lowering the temperature, and they can be dissolved by raising it. The ability to monitor the formation and melting of these crystals yields information that can be used to monitor crystal formation and growth. Here, we describe the development of an inexpensive tool that enables direct observation of the crystal growth process as a function of both time and temperature. Using the hanging-drop crystallization of the well-characterized 2-turn DNA tensegrity triangle motif for our model system, its response to temperature has been characterized visually.

Original languageEnglish (US)
Pages (from-to)1-5
Number of pages5
JournalJournal of Crystal Growth
StatePublished - Oct 15 2017


  • A2. Growth from solution
  • B1. Cyanine
  • B3. Microscope cameras

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Inorganic Chemistry
  • Materials Chemistry


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