Time lapse microscopy of temperature control during self-assembly of 3D DNA crystals

Fiona W. Conn; Michael Alexander Jong; Andre Tan; Robert Tseng; Eunice Park; Yoel P. Ohayon; Ruojie Sha; Chengde Mao; Nadrian C. Seeman

doi:10.1016/j.jcrysgro.2017.07.019

Time lapse microscopy of temperature control during self-assembly of 3D DNA crystals

Fiona W. Conn, Michael Alexander Jong, Andre Tan, Robert Tseng, Eunice Park, Yoel P. Ohayon, Ruojie Sha, Chengde Mao, Nadrian C. Seeman

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

DNA nanostructures are created by exploiting the high fidelity base-pairing interactions of double-stranded branched DNA molecules. These structures present a convenient medium for the self-assembly of macroscopic 3D crystals. In some self-assemblies in this system, crystals can be formed by lowering the temperature, and they can be dissolved by raising it. The ability to monitor the formation and melting of these crystals yields information that can be used to monitor crystal formation and growth. Here, we describe the development of an inexpensive tool that enables direct observation of the crystal growth process as a function of both time and temperature. Using the hanging-drop crystallization of the well-characterized 2-turn DNA tensegrity triangle motif for our model system, its response to temperature has been characterized visually.

Original language	English (US)
Pages (from-to)	1-5
Number of pages	5
Journal	Journal of Crystal Growth
Volume	476
DOIs	https://doi.org/10.1016/j.jcrysgro.2017.07.019
State	Published - Oct 15 2017

Keywords

A2. Growth from solution
B1. Cyanine
B3. Microscope cameras

ASJC Scopus subject areas

Condensed Matter Physics
Inorganic Chemistry
Materials Chemistry

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10.1016/j.jcrysgro.2017.07.019

Cite this

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title = "Time lapse microscopy of temperature control during self-assembly of 3D DNA crystals",

abstract = "DNA nanostructures are created by exploiting the high fidelity base-pairing interactions of double-stranded branched DNA molecules. These structures present a convenient medium for the self-assembly of macroscopic 3D crystals. In some self-assemblies in this system, crystals can be formed by lowering the temperature, and they can be dissolved by raising it. The ability to monitor the formation and melting of these crystals yields information that can be used to monitor crystal formation and growth. Here, we describe the development of an inexpensive tool that enables direct observation of the crystal growth process as a function of both time and temperature. Using the hanging-drop crystallization of the well-characterized 2-turn DNA tensegrity triangle motif for our model system, its response to temperature has been characterized visually.",

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doi = "10.1016/j.jcrysgro.2017.07.019",

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AU - Conn, Fiona W.

AU - Jong, Michael Alexander

AU - Tan, Andre

AU - Tseng, Robert

AU - Park, Eunice

AU - Ohayon, Yoel P.

AU - Sha, Ruojie

AU - Mao, Chengde

AU - Seeman, Nadrian C.

PY - 2017/10/15

Y1 - 2017/10/15

N2 - DNA nanostructures are created by exploiting the high fidelity base-pairing interactions of double-stranded branched DNA molecules. These structures present a convenient medium for the self-assembly of macroscopic 3D crystals. In some self-assemblies in this system, crystals can be formed by lowering the temperature, and they can be dissolved by raising it. The ability to monitor the formation and melting of these crystals yields information that can be used to monitor crystal formation and growth. Here, we describe the development of an inexpensive tool that enables direct observation of the crystal growth process as a function of both time and temperature. Using the hanging-drop crystallization of the well-characterized 2-turn DNA tensegrity triangle motif for our model system, its response to temperature has been characterized visually.

AB - DNA nanostructures are created by exploiting the high fidelity base-pairing interactions of double-stranded branched DNA molecules. These structures present a convenient medium for the self-assembly of macroscopic 3D crystals. In some self-assemblies in this system, crystals can be formed by lowering the temperature, and they can be dissolved by raising it. The ability to monitor the formation and melting of these crystals yields information that can be used to monitor crystal formation and growth. Here, we describe the development of an inexpensive tool that enables direct observation of the crystal growth process as a function of both time and temperature. Using the hanging-drop crystallization of the well-characterized 2-turn DNA tensegrity triangle motif for our model system, its response to temperature has been characterized visually.

KW - A2. Growth from solution

KW - B1. Cyanine

KW - B3. Microscope cameras

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JF - Journal of Crystal Growth

ER -

Time lapse microscopy of temperature control during self-assembly of 3D DNA crystals

Abstract

Keywords

ASJC Scopus subject areas

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