@article{8bea606562f84121b4f54ebabbcdffed,
title = "Tissue fluidity mediated by adherens junction dynamics promotes planar cell polarity-driven ommatidial rotation",
abstract = "The phenomenon of tissue fluidity—cells{\textquoteright} ability to rearrange relative to each other in confluent tissues—has been linked to several morphogenetic processes and diseases, yet few molecular regulators of tissue fluidity are known. Ommatidial rotation (OR), directed by planar cell polarity signaling, occurs during Drosophila eye morphogenesis and shares many features with polarized cellular migration in vertebrates. We utilize in vivo live imaging analysis tools to quantify dynamic cellular morphologies during OR, revealing that OR is driven autonomously by ommatidial cell clusters rotating in successive pulses within a permissive substrate. Through analysis of a rotation-specific nemo mutant, we demonstrate that precise regulation of junctional E-cadherin levels is critical for modulating the mechanical properties of the tissue to allow rotation to progress. Our study defines Nemo as a molecular tool to induce a transition from solid-like tissues to more viscoelastic tissues broadening our molecular understanding of tissue fluidity.",
author = "Nabila Founounou and Reza Farhadifar and Collu, {Giovanna M.} and Ursula Weber and Shelley, {Michael J.} and Marek Mlodzik",
note = "Funding Information: We are most grateful to Dan Needleman for his encouragement and help, and Hai-Yin Wu and Che-Hang Yu in the Needleman lab for allowing access to their laser ablation set up. We thank the Bloomington Stock Center, Michael Welte, and Esther Verheyen for fly strains, and the Developmental Studies Hybridoma Bank (DSHB, supported by the NICHD of the NIH and maintained at the University of Iowa), and Hugo Bellen for antibodies. We are grateful to Ivana Mirkovic for sharing unpublished results and all Mlodzik lab members for helpful input and many discussions, Jun Wu for technical advice, and C.P. Heisenberg and Benoit Dehapiot for helpful comments on the manuscript. This study was only possible thanks to the groundbreaking work of Suzanne Eaton, who has been inspirational to the field. This work was supported by NIH grants R21 HD095043 (technology development), R01 EY013256, and R35 GM127103 to MM; confocal microscopy was in part performed at the Microscopy CoRE, which is in part supported by the Tisch Cancer Institute P30 CA196521 grant from the NCI. Publisher Copyright: {\textcopyright} 2021, The Author(s).",
year = "2021",
month = dec,
doi = "10.1038/s41467-021-27253-0",
language = "English (US)",
volume = "12",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}