Tissue-specific gene expression profiling by cell sorting

Pui Leng Ip, Kenneth D. Birnbaum

Research output: Contribution to journalArticle

Abstract

Many cell populations have been labeled using stable reporters containing a fl uorescent protein. These same marker lines can be used to capture specifi c cell types or marked cell populations after a brief enzymatic digestion to dissociate tissues. Here, we describe the use of Fluorescence Activated Cell Sorting (FACS) to isolate a rare population of cells marked with Green Fluorescent Protein (GFP). The FACS is a powerful selection tool that can be used to isolate high-quality RNA while selecting for fl uorescence, cell size, and other properties.

Original languageEnglish (US)
Pages (from-to)175-183
Number of pages9
JournalMethods in Molecular Biology
Volume1284
DOIs
StatePublished - Jan 1 2015

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Keywords

  • Cell sorting
  • Cell type isolation
  • FACS
  • Plants
  • Quiescent center
  • RNA profiling

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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