Tissue-specific gene expression profiling by cell sorting

Pui Leng Ip, Kenneth D. Birnbaum

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Many cell populations have been labeled using stable reporters containing a fluorescent protein. These same marker lines can be used to capture specific cell types or marked cell populations after a brief enzymatic digestion to dissociate tissues. Here, we describe the use of Fluorescence Activated Cell Sorting (FACS) to isolate a rare population of cells marked with Green Fluorescent Protein (GFP). The FACS is a powerful selection tool that can be used to isolate high-quality RNA while selecting for fluorescence, cell size, and other properties.

Original languageEnglish (US)
Title of host publicationPlant Functional Genomics
Subtitle of host publicationMethods and Protocols: Second Edition
PublisherSpringer New York
Pages175-183
Number of pages9
ISBN (Electronic)9781493924448
ISBN (Print)9781493924431
DOIs
StatePublished - Mar 11 2015

Keywords

  • Cell sorting
  • Cell type isolation
  • FACS
  • Plants
  • Quiescent center
  • RNA profiling

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Medicine(all)

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