Tissue-specific gene expression profiling by cell sorting

Pui Leng Ip; Kenneth D. Birnbaum

doi:10.1007/978-1-4939-2444-8_8

Tissue-specific gene expression profiling by cell sorting

Pui Leng Ip, Kenneth D. Birnbaum

Biology and Genomics

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Many cell populations have been labeled using stable reporters containing a fluorescent protein. These same marker lines can be used to capture specific cell types or marked cell populations after a brief enzymatic digestion to dissociate tissues. Here, we describe the use of Fluorescence Activated Cell Sorting (FACS) to isolate a rare population of cells marked with Green Fluorescent Protein (GFP). The FACS is a powerful selection tool that can be used to isolate high-quality RNA while selecting for fluorescence, cell size, and other properties.

Original language	English (US)
Title of host publication	Plant Functional Genomics
Subtitle of host publication	Methods and Protocols: Second Edition
Publisher	Springer New York
Pages	175-183
Number of pages	9
ISBN (Electronic)	9781493924448
ISBN (Print)	9781493924431
DOIs	https://doi.org/10.1007/978-1-4939-2444-8_8
State	Published - Mar 11 2015

Keywords

Cell sorting
Cell type isolation
FACS
Plants
Quiescent center
RNA profiling

ASJC Scopus subject areas

General Agricultural and Biological Sciences
General Medicine

Access to Document

10.1007/978-1-4939-2444-8_8

Cite this

@inbook{e98addb97ee849ecaf9e179d42a44468,

title = "Tissue-specific gene expression profiling by cell sorting",

abstract = "Many cell populations have been labeled using stable reporters containing a fluorescent protein. These same marker lines can be used to capture specific cell types or marked cell populations after a brief enzymatic digestion to dissociate tissues. Here, we describe the use of Fluorescence Activated Cell Sorting (FACS) to isolate a rare population of cells marked with Green Fluorescent Protein (GFP). The FACS is a powerful selection tool that can be used to isolate high-quality RNA while selecting for fluorescence, cell size, and other properties.",

keywords = "Cell sorting, Cell type isolation, FACS, Plants, Quiescent center, RNA profiling",

author = "Ip, {Pui Leng} and Birnbaum, {Kenneth D.}",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2015. All rights are reserved.",

year = "2015",

month = mar,

day = "11",

doi = "10.1007/978-1-4939-2444-8_8",

language = "English (US)",

isbn = "9781493924431",

pages = "175--183",

booktitle = "Plant Functional Genomics",

publisher = "Springer New York",

}

TY - CHAP

T1 - Tissue-specific gene expression profiling by cell sorting

AU - Ip, Pui Leng

AU - Birnbaum, Kenneth D.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015. All rights are reserved.

PY - 2015/3/11

Y1 - 2015/3/11

N2 - Many cell populations have been labeled using stable reporters containing a fluorescent protein. These same marker lines can be used to capture specific cell types or marked cell populations after a brief enzymatic digestion to dissociate tissues. Here, we describe the use of Fluorescence Activated Cell Sorting (FACS) to isolate a rare population of cells marked with Green Fluorescent Protein (GFP). The FACS is a powerful selection tool that can be used to isolate high-quality RNA while selecting for fluorescence, cell size, and other properties.

AB - Many cell populations have been labeled using stable reporters containing a fluorescent protein. These same marker lines can be used to capture specific cell types or marked cell populations after a brief enzymatic digestion to dissociate tissues. Here, we describe the use of Fluorescence Activated Cell Sorting (FACS) to isolate a rare population of cells marked with Green Fluorescent Protein (GFP). The FACS is a powerful selection tool that can be used to isolate high-quality RNA while selecting for fluorescence, cell size, and other properties.

KW - Cell sorting

KW - Cell type isolation

KW - FACS

KW - Plants

KW - Quiescent center

KW - RNA profiling

UR - http://www.scopus.com/inward/record.url?scp=84954618907&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84954618907&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-2444-8_8

DO - 10.1007/978-1-4939-2444-8_8

M3 - Chapter

C2 - 25757772

AN - SCOPUS:84954618907

SN - 9781493924431

SP - 175

EP - 183

BT - Plant Functional Genomics

PB - Springer New York

ER -

Tissue-specific gene expression profiling by cell sorting

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this