Topical vancomycin and its effect on survival and migration of osteoblasts, fibroblasts, and myoblasts: An in vitro study

James X. Liu; Dalibel Bravo; John Buza; Thorsten Kirsch; Oran Kennedy; Andrew Rokito; Joseph D. Zuckerman; Mandeep S. Virk

doi:10.1016/j.jor.2018.01.032

Topical vancomycin and its effect on survival and migration of osteoblasts, fibroblasts, and myoblasts: An in vitro study

James X. Liu, Dalibel Bravo, John Buza, Thorsten Kirsch, Oran Kennedy, Andrew Rokito, Joseph D. Zuckerman, Mandeep S. Virk

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

The purpose of this study was to examine the influence of topical vancomycin on cell migration and survival of tissue healing cells. Human osteoblasts, myoblasts and fibroblasts were exposed to vancomycin at concentrations of 1, 3, 6, or 12 mg/cm² for either a 1-h or 48-h (continuous) duration. Continuous exposure to all vancomycin concentrations significantly reduced cell survival (<22% cells survived) and migration in osteoblasts and myoblasts (P < 0.001). 1-h vancomycin exposure reduced osteoblast and myoblast survival and migration only at 12 mg/cm² (P < 0.001). Further in vivo studies are warranted to optimize the dosage of intrawound vancomycin.

Original language	English (US)
Pages (from-to)	53-58
Number of pages	6
Journal	Journal of Orthopaedics
Volume	15
Issue number	1
DOIs	https://doi.org/10.1016/j.jor.2018.01.032
State	Published - Mar 2018

Keywords

Cell migration
Cell survival
Fibroblast
Myoblast
Osteoblast
Vancomycin

ASJC Scopus subject areas

Orthopedics and Sports Medicine

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10.1016/j.jor.2018.01.032

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title = "Topical vancomycin and its effect on survival and migration of osteoblasts, fibroblasts, and myoblasts: An in vitro study",

abstract = "The purpose of this study was to examine the influence of topical vancomycin on cell migration and survival of tissue healing cells. Human osteoblasts, myoblasts and fibroblasts were exposed to vancomycin at concentrations of 1, 3, 6, or 12 mg/cm2 for either a 1-h or 48-h (continuous) duration. Continuous exposure to all vancomycin concentrations significantly reduced cell survival (<22% cells survived) and migration in osteoblasts and myoblasts (P < 0.001). 1-h vancomycin exposure reduced osteoblast and myoblast survival and migration only at 12 mg/cm2 (P < 0.001). Further in vivo studies are warranted to optimize the dosage of intrawound vancomycin.",

keywords = "Cell migration, Cell survival, Fibroblast, Myoblast, Osteoblast, Vancomycin",

author = "Liu, {James X.} and Dalibel Bravo and John Buza and Thorsten Kirsch and Oran Kennedy and Andrew Rokito and Zuckerman, {Joseph D.} and Virk, {Mandeep S.}",

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year = "2018",

month = mar,

doi = "10.1016/j.jor.2018.01.032",

language = "English (US)",

volume = "15",

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journal = "Journal of Orthopaedics",

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T2 - An in vitro study

AU - Liu, James X.

AU - Bravo, Dalibel

AU - Buza, John

AU - Kirsch, Thorsten

AU - Kennedy, Oran

AU - Rokito, Andrew

AU - Zuckerman, Joseph D.

AU - Virk, Mandeep S.

PY - 2018/3

Y1 - 2018/3

N2 - The purpose of this study was to examine the influence of topical vancomycin on cell migration and survival of tissue healing cells. Human osteoblasts, myoblasts and fibroblasts were exposed to vancomycin at concentrations of 1, 3, 6, or 12 mg/cm2 for either a 1-h or 48-h (continuous) duration. Continuous exposure to all vancomycin concentrations significantly reduced cell survival (<22% cells survived) and migration in osteoblasts and myoblasts (P < 0.001). 1-h vancomycin exposure reduced osteoblast and myoblast survival and migration only at 12 mg/cm2 (P < 0.001). Further in vivo studies are warranted to optimize the dosage of intrawound vancomycin.

AB - The purpose of this study was to examine the influence of topical vancomycin on cell migration and survival of tissue healing cells. Human osteoblasts, myoblasts and fibroblasts were exposed to vancomycin at concentrations of 1, 3, 6, or 12 mg/cm2 for either a 1-h or 48-h (continuous) duration. Continuous exposure to all vancomycin concentrations significantly reduced cell survival (<22% cells survived) and migration in osteoblasts and myoblasts (P < 0.001). 1-h vancomycin exposure reduced osteoblast and myoblast survival and migration only at 12 mg/cm2 (P < 0.001). Further in vivo studies are warranted to optimize the dosage of intrawound vancomycin.

KW - Cell migration

KW - Cell survival

KW - Fibroblast

KW - Myoblast

KW - Osteoblast

KW - Vancomycin

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Topical vancomycin and its effect on survival and migration of osteoblasts, fibroblasts, and myoblasts: An in vitro study

Abstract

Keywords

ASJC Scopus subject areas

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