Topical vancomycin and its effect on survival and migration of osteoblasts, fibroblasts, and myoblasts: An in vitro study

James X. Liu, Dalibel Bravo, John Buza, Thorsten Kirsch, Oran Kennedy, Andrew Rokito, Joseph D. Zuckerman, Mandeep S. Virk

    Research output: Contribution to journalArticle

    Abstract

    The purpose of this study was to examine the influence of topical vancomycin on cell migration and survival of tissue healing cells. Human osteoblasts, myoblasts and fibroblasts were exposed to vancomycin at concentrations of 1, 3, 6, or 12 mg/cm2 for either a 1-h or 48-h (continuous) duration. Continuous exposure to all vancomycin concentrations significantly reduced cell survival (<22% cells survived) and migration in osteoblasts and myoblasts (P < 0.001). 1-h vancomycin exposure reduced osteoblast and myoblast survival and migration only at 12 mg/cm2 (P < 0.001). Further in vivo studies are warranted to optimize the dosage of intrawound vancomycin.

    Original languageEnglish (US)
    Pages (from-to)53-58
    Number of pages6
    JournalJournal of Orthopaedics
    Volume15
    Issue number1
    DOIs
    StatePublished - Mar 2018

    Keywords

    • Cell migration
    • Cell survival
    • Fibroblast
    • Myoblast
    • Osteoblast
    • Vancomycin

    ASJC Scopus subject areas

    • Orthopedics and Sports Medicine

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  • Cite this

    Liu, J. X., Bravo, D., Buza, J., Kirsch, T., Kennedy, O., Rokito, A., Zuckerman, J. D., & Virk, M. S. (2018). Topical vancomycin and its effect on survival and migration of osteoblasts, fibroblasts, and myoblasts: An in vitro study. Journal of Orthopaedics, 15(1), 53-58. https://doi.org/10.1016/j.jor.2018.01.032