Toward a Comprehensive Temperature-Sensitive Mutant Repository of the Essential Genes of Saccharomyces cerevisiae

Shay Ben-Aroya, Candice Coombes, Teresa Kwok, Kathryn A. O'Donnell, Jef D. Boeke, Philip Hieter

    Research output: Contribution to journalArticle

    Abstract

    The Saccharomyces cereivisiae gene deletion project revealed that approximately 20% of yeast genes are required for viability. The analysis of essential genes traditionally relies on conditional mutants, typically temperature-sensitive (ts) alleles. We developed a systematic approach (termed "diploid shuffle") useful for generating a ts allele for each essential gene in S. cerevisiae and for improved genetic manipulation of mutant alleles and gene constructs in general. Importantly, each ts allele resides at its normal genomic locus, flanked by specific cognate UPTAG and DNTAG bar codes. A subset of 250 ts mutants, including ts alleles for all uncharacterized essential genes and prioritized for genes with human counterparts, is now ready for distribution. The importance of this collection is demonstrated by biochemical and genetic screens that reveal essential genes involved in RNA processing and maintenance of chromosomal stability.

    Original languageEnglish (US)
    Pages (from-to)248-258
    Number of pages11
    JournalMolecular Cell
    Volume30
    Issue number2
    DOIs
    StatePublished - Apr 25 2008

    Keywords

    • DNA
    • RNA

    ASJC Scopus subject areas

    • Molecular Biology
    • Cell Biology

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