Towards the fluorescence resonance energy transfer(FRET) scanning near-field optical microscopy: Investigation of nanolocal FRET processes and FRET probe microscope 3

S. K. Sekatskiǐ, G. T. Shubeita, M. Chergui, G. Dietler, B. N. Mironov, D. A. Lapshin, V. S. Letokhov

Research output: Contribution to journalArticlepeer-review

Abstract

The fluorescence resonance energy-transfer(FRET) process is investigated between donor dye molecules deposited on the sample surface and acceptor dye molecules deposited on the tips of scanning near-field and atomic force microscopes. The FRET process was observed only when the tip acquired contact with the sample and took place in regions of sizes of only a few tens of nanometers with only a few thousands(or even hundreds) of molecules involved. The dependence of the FRET intensity on the tip-sample acting force is recorded and interpreted. In relation to the obtained results, the construction of a previously proposed one-atom FRET SNOM is described.

Original languageEnglish (US)
Pages (from-to)769-777
Number of pages9
JournalJournal of Experimental and Theoretical Physics
Volume90
Issue number5
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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