Tracking fluorescently labeled neurons in developing brain

Gord Fishell, Richard Blazeski, Pierre Godement, Rodolfo Rivas, L. I.Chong Wang, Carol A. Mason

Research output: Contribution to journalReview article

Abstract

For decades, time-lapse microscopy has been used to track dynamic events associated with biological phenomena. Time-lapse studies of the developing nervous system have been restricted to analysis of dissociated cell cultures or of a series of static images from living organisms. The advent of new fluorescent dyes and video imaging technology has produced novel views of the behavior of neurons in the context of the developing nervous tissue, such as migrations within and away from proliferative zones and navigation of axonal processes to synaptic targets. After fixation of the tissue preparation, time-lapse monitoring can be followed by other analytical techniques and forms of microscopy, e.g., immunocytochemistry or electron microscopy, producing information on the interactions of individual cells whose behavioral histories are known. The power of video time-lapse microscopy of living brain tissue lies in the firsthand documentation of developmental patterning, which in turn can serve as an experimental assay.

Original languageEnglish (US)
Pages (from-to)324-334
Number of pages11
JournalFASEB Journal
Volume9
Issue number5
DOIs
StatePublished - Jan 1 1995

Keywords

  • axon guidance
  • dye labeling
  • mammalian brain
  • neuronal migration
  • video time lapse microscopy

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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    Fishell, G., Blazeski, R., Godement, P., Rivas, R., Wang, L. I. C., & Mason, C. A. (1995). Tracking fluorescently labeled neurons in developing brain. FASEB Journal, 9(5), 324-334. https://doi.org/10.1096/fasebj.9.5.7896001