Tracts of A·T base pairs retard the electrophoretic mobility of short DNA duplexes

Jung Huei Chen, Nadrian C. Seeman, Neville R. Kallenbach

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An investigation of the mobility of short duplexes of DNA -octamers and decamers-on polyacrylamide gels is presented, showing that molecules containing less than one helical turn exhibit sequence dependent mobilities. Analysis of chains with different sequences indicates that any arrangement of two or more adjacent A·T base pairs causes a duplex to move more slowly than does any combination of isolated A·T pairs This behavior appears to be an intrinsic property of these sequences, since the anomaly persists in the absence of magnesium or presence of spermine and is not due to strand dissociation. In two decamers we studied, the position of A·T tracts within a duplex can be shown to influence mobility: the sequence GA4T4C associated with bending or curvature of the helix axis when ligated into polymers migrates more slowly than the corresponding sequence GT4A4C polymers of which migrate as linear B DNA.

Original languageEnglish (US)
Pages (from-to)6803-6811
Number of pages9
JournalNucleic acids research
Issue number14
StatePublished - Jul 25 1988

ASJC Scopus subject areas

  • Genetics


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