TY - JOUR
T1 - Transcription and DNA adducts
T2 - What happens when the message gets cut off?
AU - Scicchitano, David A.
AU - Olesnicky, Eugenia C.
AU - Dimitri, Alexandra
N1 - Funding Information:
The authors thank all the members of the lab for their help with this manuscript. We also thank Dr. Rebecca Perlow and Professor Suse Broyde for valuable discussion. This work was supported by NIH grant ES10581 to D.A.S.
PY - 2004/12/2
Y1 - 2004/12/2
N2 - DNA damage located within a gene's transcription unit can cause RNA polymerase to stall at the modified site, resulting in a truncated transcript, or progress past, producing full-length RNA. However, it is not immediately apparent why some lesions pose strong barriers to elongation while others do not. Studies using site-specifically damaged DNA templates have demonstrated that a wide range of lesions can impede the progress of elongating transcription complexes. The collected results of this work provide evidence for the idea that subtle structural elements can influence how an RNA polymerase behaves when it encounters a DNA adduct during elongation. These elements include: (1) the ability of the RNA polymerase active site to accommodate the damaged base; (2) the size and shape of the adduct, which includes the specific modified base; (3) the stereochemistry of the adduct; (4) the base incorporated into the growing transcript; and (5) the local DNA sequence.
AB - DNA damage located within a gene's transcription unit can cause RNA polymerase to stall at the modified site, resulting in a truncated transcript, or progress past, producing full-length RNA. However, it is not immediately apparent why some lesions pose strong barriers to elongation while others do not. Studies using site-specifically damaged DNA templates have demonstrated that a wide range of lesions can impede the progress of elongating transcription complexes. The collected results of this work provide evidence for the idea that subtle structural elements can influence how an RNA polymerase behaves when it encounters a DNA adduct during elongation. These elements include: (1) the ability of the RNA polymerase active site to accommodate the damaged base; (2) the size and shape of the adduct, which includes the specific modified base; (3) the stereochemistry of the adduct; (4) the base incorporated into the growing transcript; and (5) the local DNA sequence.
KW - DNA damage
KW - DNA repair
KW - Transcription
KW - Transcription-coupled repair
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U2 - 10.1016/j.dnarep.2004.06.004
DO - 10.1016/j.dnarep.2004.06.004
M3 - Review article
C2 - 15474416
AN - SCOPUS:5044227858
SN - 1568-7864
VL - 3
SP - 1537
EP - 1548
JO - DNA Repair
JF - DNA Repair
IS - 12
ER -