Transcription-Replication Conflicts as a Source of Genome Instability

Liana Goehring, Tony T. Huang, Duncan J. Smith

Research output: Contribution to journalReview articlepeer-review


Transcription and replication both require large macromolecular complexes to act on a DNA template, yet these machineries cannot simultaneously act on the same DNA sequence. Conflicts between the replication and transcription machineries (transcription-replication conflicts, or TRCs) are widespread in both prokaryotes and eukaryotes and have the capacity to both cause DNA damage and compromise complete, faithful replication of the genome. This review will highlight recent studies investigating the genomic locations of TRCs and the mechanisms by which they may be prevented, mitigated, or resolved. We address work from both model organisms and mammalian systems but predominantly focus on multicellular eukaryotes owing to the additional complexities inherent in the coordination of repli-cation and transcription in the context of cell type-specific gene expression and higher-order chromatin organization.

Original languageEnglish (US)
Pages (from-to)157-179
Number of pages23
JournalAnnual Review of Genetics
StatePublished - Nov 27 2023


  • DNA repair
  • RNA polymerase
  • TRCs
  • genomic instability
  • origin firing
  • replication stress
  • replisome
  • transcription
  • transcription-replication conflicts

ASJC Scopus subject areas

  • Genetics


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