Abstract
Transforming growth factor (TGF)-β1, a crucial molecule in metastatic bone cancer, stimulates collagenase-3 expression in the human breast cancer cell line, MDA-MB231. Cycloheximide inhibited this stimulation, indicating that de novo protein synthesis was essential for this response. We examined whether mitogen-activated protein kinase (MAPK) and/or Smad pathways are involved in TGF-β1-stimulated collagenase-3 expression in MDA-MB231 cells. Biochemical blockade of extracellular regulated kinase-1/2 and p38 MAPK pathways partially abolished TGF-β1-stimulated collagenase-3 mRNA expression; whereas overexpression of a dominant negative form of Smad3 completely blocked the TGF-β1-response. These data indicate that TGF-β1-induced MAPK and Smad pathways are involved in TGF-β1-stimulated collagenase-3 expression in MDA-MB231 cells.
Original language | English (US) |
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Pages (from-to) | 31-35 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 532 |
Issue number | 1-2 |
DOIs | |
State | Published - Dec 4 2002 |
Keywords
- Breast cancer metastasis
- Collagenase-3
- Extracellular matrix
- Transforming growth factor-β1 signaling
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology