Transcriptional elongation by RNA polymerase II is stimulated by transactivators

Krassimir Yankulov, Justin Blau, Tracey Purton, Sadia Roberts, David L. Bentley

Research output: Contribution to journalArticlepeer-review

Abstract

We report that a variety of transactivators stimulate elongation by RNA polymerase II. Activated transcription complexes have high processivity and are able to read through pausing and termination sites efficiently. In contrast, nonactivated and "squelched" transcription mostly arrests prematurely. Activators differ in the extent to which they stimulate processivity; for example, GAL4-VP16 and GAL4-E1a are more effective than GAL4-AH. The stimulation of elongation can be as important as the stimulation of initiation in activating expression of a reporter gene. We suggest that setting the competence of polymerase II to elongate is an integral part of the initiation step that is controlled by activators cooperating with the general transcription factors.

Original languageEnglish (US)
Pages (from-to)749-759
Number of pages11
JournalCell
Volume77
Issue number5
DOIs
StatePublished - Jun 3 1994

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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