Transcriptional profile of the arabidopsis root quiescent center

Tal Nawy, Ji Young Lee, Juliette Colinas, Jean Y. Wang, Sumena C. Thongrod, Jocelyn E. Malamy, Kenneth Birnbaum, Philip N. Benfey

Research output: Contribution to journalArticle

Abstract

The self-renewal characteristics of stem cells render them vital engines of development. To better understand the molecular mechanisms that determine the properties of stem cells, transcript profiling was conducted on quiescent center (QC) cells from the Arabidopsis thaliana root meristem. The AGAMOUS-LIKE 42 (AGL42) gene, which encodes a MADS box transcription factor whose expression is enriched in the QC, was used to mark these cells. RNA was isolated from sorted cells, labeled, and hybridized to Affymetrix microarrays. Comparisons with digital in situ expression profiles of surrounding tissues identified a set of genes enriched in the QC. Promoter regions from a subset of transcription factors identified as enriched in the QC conferred expression in the QC. These studies demonstrated that it is possible to successfully isolate and profile a rare cell type in the plant. Mutations in all enriched transcription factor genes including AGL42 exhibited no detectable root phenotype, raising the possibility of a high degree of functional redundancy in the QC.

Original languageEnglish (US)
Pages (from-to)1908-1925
Number of pages18
JournalPlant Cell
Volume17
Issue number7
DOIs
StatePublished - 2005

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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    Nawy, T., Lee, J. Y., Colinas, J., Wang, J. Y., Thongrod, S. C., Malamy, J. E., Birnbaum, K., & Benfey, P. N. (2005). Transcriptional profile of the arabidopsis root quiescent center. Plant Cell, 17(7), 1908-1925. https://doi.org/10.1105/tpc.105.031724