Transcriptional regulation of Rab32/38, a specific marker of pigment cell formation in Ciona robusta

Claudia Racioppi, Ugo Coppola, Lionel Christiaen, Filomena Ristoratore

Research output: Contribution to journalArticle

Abstract

Through a myriad of pigments stored in different cells, animal pigmentation represents a crucial process to face disparate environmental and ecological challenges. In vertebrates, the small GTPase Rab32 and Rab38 have a conserved role in the transport of key melanogenic enzymes, as tyrosinase (tyr) and tyrosinase-related protein (tyrp), to the melanosomes in formation. We provide a survey on Rab32/38 evolution and its regulatory logics during pigment cell formation in Ciona robusta. Our phylogeny supports the existence of a single Rab32/38 gene in tunicates, which is probably the unique transporter for tyrosinase family members in this clade. Different deletions allow us to identify the minimal cis-regulatory element able to recapitulate the endogenous gene expression during pigment cell development in C. robusta. In this conserved region, we identified two putative binding sites for the transcription factor Mitf, which is known for its role as regulator of pigmentation in vertebrates. Mutational analysis revealed that both Mitf binding sites are essential for the activity of this regulatory region and we demonstrated that Mitf misexpression is able to induce ectopic activation of the Rab32/38 regulatory region in vivo. Our results strongly indicate that Mitf is involved in the regulation of Rab32/38 activity during Ciona pigment cell development.

Original languageEnglish (US)
Pages (from-to)111-118
Number of pages8
JournalDevelopmental Biology
Volume448
Issue number2
DOIs
StatePublished - Apr 15 2019

Keywords

  • GTPase
  • Mitf
  • Pigmentation
  • Rab32/38
  • Transcription factors
  • Tunicates

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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