Transcriptome Analysis Identified Gene Regulation Networks in Soybean Leaves Perturbed by the Coronatine Toxin

Xiong Zhang; Bin He; Sheng Sun; Zhipeng Zhang; Tian Li; Hehe Wang; Zhicheng Liu; Ahmed Jawaad Afzal; Xueqing Geng

doi:10.3389/fsufs.2021.663238

Transcriptome Analysis Identified Gene Regulation Networks in Soybean Leaves Perturbed by the Coronatine Toxin

Xiong Zhang, Bin He, Sheng Sun, Zhipeng Zhang, Tian Li, Hehe Wang, Zhicheng Liu, Ahmed Jawaad Afzal, Xueqing Geng

Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The non-host specific Pseudomonas syringae phytotoxin Coronatine (COR) causes chlorosis and promotes toxicity by inducing physiological changes in plants. We performed transcriptome analysis to better understand plants' transcriptional and metabolic response to COR. Toward this end, mock-treated and COR-treated soybean plants were analyzed by RNA-Seq. A total of 4,545 genes were differentially expressed between the two treatments, of which 2,170 were up-regulated whereas 2,375 were down-regulated in COR treated samples. Gene annotation and pathway analysis conducted using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) databases revealed that the differential genes were involved in photosynthesis, jasmonic acid (JA) synthesis, signal transduction, and phenylpropane metabolism. This study will provide new insights into COR mediated responses and extend our understanding of COR function in plants.

Original language	English (US)
Article number	663238
Journal	Frontiers in Sustainable Food Systems
Volume	5
DOIs	https://doi.org/10.3389/fsufs.2021.663238
State	Published - Apr 19 2021

Keywords

Pseudomonas syringae
RNA-Seq
coronatine
differentially expressed genes
soybean

ASJC Scopus subject areas

Global and Planetary Change
Food Science
Ecology
Agronomy and Crop Science
Management, Monitoring, Policy and Law
Horticulture

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10.3389/fsufs.2021.663238

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title = "Transcriptome Analysis Identified Gene Regulation Networks in Soybean Leaves Perturbed by the Coronatine Toxin",

abstract = "The non-host specific Pseudomonas syringae phytotoxin Coronatine (COR) causes chlorosis and promotes toxicity by inducing physiological changes in plants. We performed transcriptome analysis to better understand plants' transcriptional and metabolic response to COR. Toward this end, mock-treated and COR-treated soybean plants were analyzed by RNA-Seq. A total of 4,545 genes were differentially expressed between the two treatments, of which 2,170 were up-regulated whereas 2,375 were down-regulated in COR treated samples. Gene annotation and pathway analysis conducted using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) databases revealed that the differential genes were involved in photosynthesis, jasmonic acid (JA) synthesis, signal transduction, and phenylpropane metabolism. This study will provide new insights into COR mediated responses and extend our understanding of COR function in plants.",

keywords = "Pseudomonas syringae, RNA-Seq, coronatine, differentially expressed genes, soybean",

author = "Xiong Zhang and Bin He and Sheng Sun and Zhipeng Zhang and Tian Li and Hehe Wang and Zhicheng Liu and Afzal, {Ahmed Jawaad} and Xueqing Geng",

note = "Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Zhang, He, Sun, Zhang, Li, Wang, Liu, Afzal and Geng.",

year = "2021",

month = apr,

day = "19",

doi = "10.3389/fsufs.2021.663238",

language = "English (US)",

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AU - Zhang, Xiong

AU - He, Bin

AU - Sun, Sheng

AU - Zhang, Zhipeng

AU - Li, Tian

AU - Wang, Hehe

AU - Liu, Zhicheng

AU - Afzal, Ahmed Jawaad

AU - Geng, Xueqing

PY - 2021/4/19

Y1 - 2021/4/19

N2 - The non-host specific Pseudomonas syringae phytotoxin Coronatine (COR) causes chlorosis and promotes toxicity by inducing physiological changes in plants. We performed transcriptome analysis to better understand plants' transcriptional and metabolic response to COR. Toward this end, mock-treated and COR-treated soybean plants were analyzed by RNA-Seq. A total of 4,545 genes were differentially expressed between the two treatments, of which 2,170 were up-regulated whereas 2,375 were down-regulated in COR treated samples. Gene annotation and pathway analysis conducted using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) databases revealed that the differential genes were involved in photosynthesis, jasmonic acid (JA) synthesis, signal transduction, and phenylpropane metabolism. This study will provide new insights into COR mediated responses and extend our understanding of COR function in plants.

AB - The non-host specific Pseudomonas syringae phytotoxin Coronatine (COR) causes chlorosis and promotes toxicity by inducing physiological changes in plants. We performed transcriptome analysis to better understand plants' transcriptional and metabolic response to COR. Toward this end, mock-treated and COR-treated soybean plants were analyzed by RNA-Seq. A total of 4,545 genes were differentially expressed between the two treatments, of which 2,170 were up-regulated whereas 2,375 were down-regulated in COR treated samples. Gene annotation and pathway analysis conducted using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) databases revealed that the differential genes were involved in photosynthesis, jasmonic acid (JA) synthesis, signal transduction, and phenylpropane metabolism. This study will provide new insights into COR mediated responses and extend our understanding of COR function in plants.

KW - Pseudomonas syringae

KW - RNA-Seq

KW - coronatine

KW - differentially expressed genes

KW - soybean

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Transcriptome Analysis Identified Gene Regulation Networks in Soybean Leaves Perturbed by the Coronatine Toxin

Abstract

Keywords

ASJC Scopus subject areas

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