Transformation by simian virus 40 of spleen cells from a hyperimmune rabbit: demonstration of production of specific antibody to the immunizing antigen

A. D. Strosberg, J. J. Collins, P. H. Black, D. Malamud, S. Wilbert, K. J. Bloch, E. Haber

Research output: Contribution to journalArticlepeer-review

Abstract

Extracts of spleen cells obtained from a rabbit hyperimmunized with Type III pneumococcal vaccine and transformed with simian virus 40, were subjected to agarose gel electrophoresis in the presence of an [125I]S3 polysaccharide protein conjugate. Binding of radioactivity in the gamma globulin region was observed. Extracts and media obtained from labeled cell cultures contained a protein that bound to an S3, but not to an S8 or inactivated S3 immunoadsorbent. After elution, the bound protein showed a single band on isoelectric focusing which corresponded in isoelectric point to one of the serum anti S3 antibodies of the donor rabbit. These observations strongly suggest that a normal, committed lymphoid cell may be brought into continuous culture by virus transformation and yet retain its ability to synthesize specific antibody.

Original languageEnglish (US)
Pages (from-to)263-264
Number of pages2
JournalUnknown Journal
Volume71
Issue number2
DOIs
StatePublished - 1974

ASJC Scopus subject areas

  • General

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