TY - JOUR
T1 - Transformation by simian virus 40 of spleen cells from a hyperimmune rabbit
T2 - demonstration of production of specific antibody to the immunizing antigen
AU - Strosberg, A. D.
AU - Collins, J. J.
AU - Black, P. H.
AU - Malamud, D.
AU - Wilbert, S.
AU - Bloch, K. J.
AU - Haber, E.
PY - 1974
Y1 - 1974
N2 - Extracts of spleen cells obtained from a rabbit hyperimmunized with Type III pneumococcal vaccine and transformed with simian virus 40, were subjected to agarose gel electrophoresis in the presence of an [125I]S3 polysaccharide protein conjugate. Binding of radioactivity in the gamma globulin region was observed. Extracts and media obtained from labeled cell cultures contained a protein that bound to an S3, but not to an S8 or inactivated S3 immunoadsorbent. After elution, the bound protein showed a single band on isoelectric focusing which corresponded in isoelectric point to one of the serum anti S3 antibodies of the donor rabbit. These observations strongly suggest that a normal, committed lymphoid cell may be brought into continuous culture by virus transformation and yet retain its ability to synthesize specific antibody.
AB - Extracts of spleen cells obtained from a rabbit hyperimmunized with Type III pneumococcal vaccine and transformed with simian virus 40, were subjected to agarose gel electrophoresis in the presence of an [125I]S3 polysaccharide protein conjugate. Binding of radioactivity in the gamma globulin region was observed. Extracts and media obtained from labeled cell cultures contained a protein that bound to an S3, but not to an S8 or inactivated S3 immunoadsorbent. After elution, the bound protein showed a single band on isoelectric focusing which corresponded in isoelectric point to one of the serum anti S3 antibodies of the donor rabbit. These observations strongly suggest that a normal, committed lymphoid cell may be brought into continuous culture by virus transformation and yet retain its ability to synthesize specific antibody.
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U2 - 10.1073/pnas.71.2.263
DO - 10.1073/pnas.71.2.263
M3 - Article
C2 - 4150021
AN - SCOPUS:0015994734
VL - 71
SP - 263
EP - 264
JO - Theoretical Computer Science
JF - Theoretical Computer Science
SN - 0304-3975
IS - 2
ER -