Abstract
Extracts of spleen cells obtained from a rabbit hyperimmunized with Type III pneumococcal vaccine and transformed with simian virus 40, were subjected to agarose gel electrophoresis in the presence of an [125I]S3 polysaccharide protein conjugate. Binding of radioactivity in the gamma globulin region was observed. Extracts and media obtained from labeled cell cultures contained a protein that bound to an S3, but not to an S8 or inactivated S3 immunoadsorbent. After elution, the bound protein showed a single band on isoelectric focusing which corresponded in isoelectric point to one of the serum anti S3 antibodies of the donor rabbit. These observations strongly suggest that a normal, committed lymphoid cell may be brought into continuous culture by virus transformation and yet retain its ability to synthesize specific antibody.
Original language | English (US) |
---|---|
Pages (from-to) | 263-264 |
Number of pages | 2 |
Journal | Unknown Journal |
Volume | 71 |
Issue number | 2 |
DOIs | |
State | Published - 1974 |
ASJC Scopus subject areas
- General