Transforming growth factor β recruits persistent MAPK signaling to regulate long-term memory consolidation in Aplysia californica

Justin Shobe, Gary T. Philips, Thomas J. Carew

Research output: Contribution to journalArticle

Abstract

In this study, we explore the mechanistic relationship between growth factor signaling and kinase activity that supports the protein synthesis-dependent phase of long-term memory (LTM) consolidation for sensitization of Aplysia. Specifically, we examine LTM for tail shock-induced sensitization of the tail-elicited siphon withdrawal (T-SW) reflex, a form of memory that requires both (i) extracellular signal-regulated kinase (ERK1/2; MAPK) activity within identified sensory neurons (SNs) that mediate the T-SW and (ii) the activation of transforming growth factor β (TGFβ) signaling. We now report that repeated tail shocks that induce intermediate-term (ITM) and LTM for sensitization, also induce a sustained post-training phase of MAPK activity in SNs (lasting at least 1 h). We identified two mechanistically distinct phases of post-training MAPK: (i) an immediate phase that does not require ongoing protein synthesis or TGFβ signaling, and (ii) a sustained phase that requires both protein synthesis and extracellular TGFβ signaling. We find that LTM consolidation requires sustained MAPK, and is disrupted by inhibitors of protein synthesis and TGFβ signaling during the consolidation window. These results provide strong evidence that TGFβ signaling sustains MAPK activity as an essential mechanistic step for LTM consolidation.

Original languageEnglish (US)
Pages (from-to)182-188
Number of pages7
JournalLearning and Memory
Volume23
Issue number5
DOIs
StatePublished - May 2016

ASJC Scopus subject areas

  • Neuropsychology and Physiological Psychology
  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Transforming growth factor β recruits persistent MAPK signaling to regulate long-term memory consolidation in Aplysia californica'. Together they form a unique fingerprint.

  • Cite this