Transposon mutagenesis: Cloning of chromosomal DNA from the site of Tn916 insertion using polymerase chain reaction

Jan Novák, Lea Novak, Ging R. Shah, Wendy A. Woodruff, Page W. Caufield

Research output: Contribution to journalArticlepeer-review

Abstract

A protocol that allows fast recovery and further analyses of chromosomal DNA adjacent to the Tn916 site of insertion is described. The procedure is based on single specific primer PCR amplification using restricted chromosomal DNA ligated into a suitable vector. Two primers, one Tn916-specific and the second vector-specific, allow amplification of the chromosomal DNA flanking the site of insertion.

Original languageEnglish (US)
Pages (from-to)51-54
Number of pages4
JournalBiotechnology Techniques
Volume11
Issue number1
DOIs
StatePublished - 1997

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology

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