@article{2c7967784ad54413922e70dee2db2318,
title = "Transposon mutagenesis: Cloning of chromosomal DNA from the site of Tn916 insertion using polymerase chain reaction",
abstract = "A protocol that allows fast recovery and further analyses of chromosomal DNA adjacent to the Tn916 site of insertion is described. The procedure is based on single specific primer PCR amplification using restricted chromosomal DNA ligated into a suitable vector. Two primers, one Tn916-specific and the second vector-specific, allow amplification of the chromosomal DNA flanking the site of insertion.",
author = "Jan Nov{\'a}k and Lea Novak and Shah, {Ging R.} and Woodruff, {Wendy A.} and Caufield, {Page W.}",
note = "Funding Information: We thank David Pritchard for critical review of the manuscript. This work was supported by the NIH grant DE09082. We are grateful to Stevenson Craig and Beatrice Hahn (University of Alabama at Birmingham) for automated sequencing. DNA analysis employed GCG software package and was supported by Center for AIDS Research (P30 AI27767). Oligonucleotides were synthesized in the Cancer Center DNA Synthesis Core Facility at UAB (supported by Public Health Service Grant CA13148).",
year = "1997",
doi = "10.1007/BF02764452",
language = "English (US)",
volume = "11",
pages = "51--54",
journal = "Biotechnology Techniques",
issn = "0951-208X",
publisher = "Springer Science and Business Media B.V.",
number = "1",
}